中國醫藥大學機構典藏 China Medical University Repository, Taiwan:Item 310903500/32604
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    題名: ä¸‰é»žï¤Šè‰æŠ—氧化及鎮痛抗發炎研究
    Study on the antioxidant, antinociceptive and anti-iflammatory activities of Desmodium triflorum DC.
    作者: è³´å°šå¿—;Shang-Chih Lai
    貢獻者: è—¥å­¸é™¢ä¸­åœ‹è—¥å­¸ç ”究所博士班
    關鍵詞: ä¸‰é»žï¤Šè‰;抗氧化活性;抗發炎;鎮痛;Desmodium triflorum;Antioxidant activity;Anti-Inflammation;Analgesia
    日期: 2010
    上傳時間: 2010-09-29 14:09:33 (UTC+8)
    摘要: ä¸‰é»žï¤Šè‰ç‚ºè±†ç§‘植物三點金草 (Desmodium triflorum (L.) DC)的全
    草，為台灣民間常見之藥用植物。本研究探討三點金草之藥理活性、抗氧化效價及清除DPPH自由基儀器新應用，在藥理活性方面以鎮痛、抗發炎及保肝試驗作為評價。三點金草對醋酸扭體及福馬林舔足試驗所引起之疼痛皆具有抑制作用；在抗發炎方面，測定λ-carrageenan誘導後第3小時小鼠浮腫足蹠組織之丙二醛(MDA)、NO、TNF-α、IL1-?瓵@度及肝組織之抗氧化酵素(SOD、GSH-Rd、GSH-Px)之活性之含量變化。實驗結果顯示三點金草抗發炎作用與抗氧化活性有關，經由抑制脂質過氧化作用及提昇肝臟中酵素SOD及GSH-Rd濃度，進而降低NO、TNF-α、IL1-?瓵@度，減少氧化壓力所造成的組織及細胞的損傷，以達到抗發炎的作用，但其對於四氯化碳誘導急性肝損傷並不具保護作用。在化學抗氧化活性方面，以DPPH自由基清除試驗、TEAC、總原力試驗、總多酚及總黃酮含量測定來評價抗氧化活性，實驗結果顯示，三點金草所具有的抗氧化活性，與其所含多酚類含量有關。本研究更以清除DPPH自由基為基礎，結合影像處理軟體開發新的分析方法。而三點金草是個具有抗氧化、鎮痛及抗發炎藥理活性，三點金草在台灣民間藥用上確有其使用上的價值，且具有進一步開發成新藥的潛力。

    Desmodium triflorum D.C. (Fabaceae) is a medicinal plant in Taiwan. In this study, we evaluated the analgesic effect of the methanol extract of D. triflorum (MDT) by using animal models of acetic acid-induced writhing response and formalin test. The anti-inflammatory effect of D. triflorum was investigated by λ-carrageenan-induced paw edema in mice. We detected the activities of glutathione peroxidase (GPx) and glutathione reductase (GRd) in the liver, the levels of interleukin-1β (IL-1β), tumor necrosis factor (TNF-α), malondialdehyde (MDA) and nitric oxide (NO) in the edema paw tissues. In the analgesic test, D. triflorum decreased the acetic acid-induced writhing response and licking time in the late phase of the formalin test. In the anti-inflammatory test, D. triflorum also decreased the paw edema due to λ-carrageenan administration. Furthermore, MDT increased the activities of SOD and GRd in liver tissues and decreased MDA level in the edema paws at the 3rd hour after λ-carrageenan-induced inflammation. D. triflorum also affected the levels of interleukin-1β, tumor necrosis factor-α, NO and MDA which were induced by λ-carrageenan. The anti-inflammatory mechanism of D. triflorum might be related to the decreases in the level of MDA in the edema paw via increasing the activities of SOD and GRd in the liver, and the NO level via regulating the IL-1β production and the level of TNF-α in the inflamed tissues. Our study also found that D. triflorum did not have significant hepatoprotective activity.

    This study evaluated the antioxidant and antiproliferative activities of the crude extract and fractions of D. triflorum. In this study, the total phenolic content, 1,1-diphenyl-2-picrylhydrazyl hydrate (DPPH) free radical scavenging activity, trolox equivalent antioxidant capacity (TEAC), reducing power, total flavonoid content of D. triflorum were evaluated for the exploration of its antioxidant activities. Furthermore, its antiproliferative activities were investigated through the MTT method. The above experimental data indicated that D. triflorum was a potent antioxidant medicinal plant, and such efficacy may be mainly attributed to its polyphenolic compounds. This paper developed a new analytical method for the evaluation of antioxidant activity using 1,1-diphenyl-2-picrylhydrazyl hydrate (DPPH) and digital processing method. In conclusion, D. triflorum is an important medicinal plant in Taiwan that is worth investigating for the development of new medicines in the future.
    顯示於類別:[中國醫學研究所] 博碩士論文

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