Histidine和Carnosine對攝取高飽和油脂飲食老鼠的影響

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Title:	Histidine和Carnosine對攝取高飽和油脂飲食老鼠的影響 Effects of Histidine and Carnosine on mice with High Saturated Fat Diet
Authors:	施穎;Ying Shih
Contributors:	健康照護學院營養學系碩士班
Keywords:	組胺酸;肌肽飽和脂肪;總膽固醇;低密度脂蛋白;histidine;carnosine;saturated fat;TC (Total Cholesterol);LDL (Low Density Lipoprotein)
Date:	2010
Issue Date:	2010-09-29 12:09:45 (UTC+8)
Abstract:	Histidine和其衍生物carnosine存在於腦、骨骼肌、肝臟之中，具有抗氧化、抗發炎、幫助神經傳導和緩衝調和等生理活性。奶油主要為短鏈的飽和脂肪酸，過度攝取飽和脂肪會上升體內的血膽固醇、LDL等，嚴重時會引起肥胖和心血管相關疾病。本實驗主要探討histidine及carnosine對於攝取飽和飲食老鼠的影響，將5週齡 C57BL/6JNarl小鼠60隻，隨機分成四組，分別為 (1)正常飲食＋一般飲水 (2)飲食含5%奶油＋一般飲水 (3)飲食含5%奶油＋飲水含1 g/L histidine (4)飲食含5%奶油＋飲水含1 g/L carnosine。餵食八週後，犧牲並取血清、肝臟和心臟進行氧化傷害、發炎和脂質的分析，測量GSH含量、Antioxidant含量、SOD activity、GPx activity、TG、TC、LDL，和發炎指標：IL-6、TNF-a。結果顯示：餵食含5%奶油的飼料後，小鼠的副睪重和肝臟重均顯著上升，血清中LDL、TC，肝臟TG均顯著上升，肝中的SOD activity、GPx activity和GSH、Antioxidant含量則明顯降低；而發炎相關的細胞激素方面，肝臟和心臟的IL-6和TNF-?捖?顯著提高。但是，攝取1 g/L histidine或1 g/L carnosine飲水之小鼠，其副睪重和肝臟重都顯著減輕；而肝臟TG和血清LDL 、TC明顯降低；肝臟和心臟中IL-6和TNF-a也都有顯著下降。抗氧化的部份則顯示肝臟中的SOD及GPx activity和GSH、Antioxidant含量都有顯著的升高。實驗結果支持histidine或carnosine可減緩小鼠脂肪的累積和抗氧化、抑制發炎的效果。 Histidine and carnosine (beta-alanyl-l-histidine) are endogenously synthesized peptides present in brain, skeletal muscle, and liver. Our present study examined the effects of histidine and carnosine in mice consumed 5% butter. Mice were divided into 4 groups: normal diet with water, butter with water, histidine, or carnosine for 5 weeks. Results indicated that the intake of butter caused an increase in body weight, epididylmal fat weight, IL-6, TNF-a, LDL, TG and total cholesterol; and decrease in hepatic GSH level and activity of GPx and SOD. Intake of histidine or carnosine at 1 g/L, however, significantly reduced oxidative stress by increasing the level or activity of GSH, GPx and SOD in liver. Histidine or carnosine also appeared to significantly diminish the formations of IL-6, TNF-a, LDL, TG and total cholesterol. Based on the observed anti-oxidative, anti-inflammatory, and anti-obesity effects, the supplements of these agents might be able to attenuate the risk of obesity.
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