Objectives: The risks of cardiovascular diseases in post-menopausal women were increased. Oxidative stress is one of important contributory factors to the etiology of many cardiovascular diseases. Diosgenin, a member of steroidal sapogenin, structurally similar to estrogen has been shown to have antioxidant effects. The study investigate whether diosgenin directly prevent oxidation-induced cardiomyocyte apoptosis.
Methods: Apoptotic pathways and mitochondria membrane stabilizing potential were measured in H9c2 cardiomyoblast cells and neonatal cardiomyocytes cultured in serum-free medium for 12 h then pretreated diosgenin for 1 h, subsequently stimulated with H2O2.
Results: In this study, H2O2 treated H9c2 cardiomyoblast cells induced cytotoxicity and apoptosis by activated Fas-dependent and mitochondria-dependent pathway. And it also induced instability of mitochondria membrane potential. Further experiment results demonstrated that diosgenin protected H2O2 induced H9c2 cell apoptosis through activated survival pathway (IGF1 signaling) and recovered mitochondria membrane stabilizing potential to suppress Fas-dependent, mitochondria-dependent pathway against apoptosis. And it also inhibited the H2O2-induced cytotoxicity and apoptosis through the estrogen receptors activated phosphorylation of PI3K/Akt and extracellular regulated protein kinases 1/2 (ERK1/2) in myocardial cells.
Conclusion: Diosgenin prevented oxidation-induced cardiomyocytes cytotoxicity and apoptosis.
Objectives: Oxidative stress is one of important contributory factors to the etiology of many cardiovascular diseases. Rhodiola widely used for its high capacity to increase the organism resistance to different oxidative stress conditions. Salidroside, a phenylpropanoid glycoside separated from a traditional Chinese medicinal plant Rhodiola rosea, and has a potent antioxidant property.
Methods: Cell viability and apoptotic pathways and were measured in H9c2 cardiomyoblast cells cultured in serum-free medium for 12 h then stimulated with H2O2 for 4 h subsequently post-treated Rhodiola (salidroside) 24 h.
Results: In this study, Rhodiola (salidroside) protected H2O2 induced cytotoxicity and apoptosis through IGF1R activated extracellular regulated protein kinases 1/2 (ERK1/2) to inhibited Fas-dependent, HIF-1 alpha, Bax and Bak related apoptotic proteins in H9c2 cells.
