Inhibition of N-acetyltransferase activity and gene expression in human colon cancer cell lines by diallyl sulfide

中國醫藥大學機構典藏 China Medical University Repository, Taiwan > 生命科學院 > 生物科技學系暨碩士班 > 期刊論文 > Item 310903500/3237

jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ir.cmu.edu.tw/ir/handle/310903500/3237

题名:	Inhibition of N-acetyltransferase activity and gene expression in human colon cancer cell lines by diallyl sulfide
作者:	鍾景光(Jing-Gung Chung);(H.F. Lu);(C.C Yeh);(K.C. Cheng);(S.S. Lin);(J.H. Lee)
贡献者:	生命科學院生物科技學系;中國附醫醫學研究部
关键词:	N-Acetyltransferase;Diallyl sulfide (DAS);Human colon cancer cell lines;2-Aminofluorene
日期:	2004-02
上传时间:	2009-08-20 18:11:44 (UTC+8)
摘要:	Diallyl sulfide (DAS) is one of the major components of garlic (Allium sativum) and is widely used in the world for food. In this study, DAS was selected for testing the inhibition of arylamine N-acetyltransferase (NAT) activity (N-acetylation of 2-aminofluorene) and gene expresseion (mRNA NAT) in human colon cancer cell lines (colo 205, colo 320 DM and colo 320 HSR). The NAT activity was examined by high preformance liquid chromatography and indicated that a 24 h DAS treatment decreases Nacetylation of 2-aminofluorene in three colon (colo 205, 320 DM and colo 320 HSR) cancer cell lines. The NAT enzymes (protein) were analyzed by western blotting and flow cytometry and it indicated that DAS decreased the levels of NAT in three colon (colo 205, 320 DM and colo 320 HSR) cancer cell lines. The gene expression of NAT (mRNAT NAT) was determined by polymerase chain reaction (PCR), it was shown that DAS affect mRNA NAT expression in examined human colon cancer cell lines. This report is the first to demonstrate that DAS does inhibit human colon cancer cell NAT activity and gene expression.
關聯:	FOOD AND CHEMICAL TOXICOLOGY 42(2):195~202
显示于类别:	[生物科技學系暨碩士班] 期刊論文

文件中的档案:

档案	大小	格式	浏览次数
	0Kb	Unknown	536	检视/开启

在CMUR中所有的数据项都受到原著作权保护.

TAIR相关文章

数据加载中.....