Proliferation- and migration- enhancing effects of Ginseng and Ginsenoside Rg1 through IGF-I- and FGF-2-signaling pathways on RSC96 Schwann Cells

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Title:	Proliferation- and migration- enhancing effects of Ginseng and Ginsenoside Rg1 through IGF-I- and FGF-2-signaling pathways on RSC96 Schwann Cells
Authors:	呂明進(Ming-Chin Lu);賴東淵(Tung-Yuan Lai);(Jin-Ming Hwang);陳賢德(Hsien-Te Chen);(Sheng-Huang Chang);蔡輔仁(Fuu-Jen Tsai);(Hwai-Lee Wang);林建仲(Chien-Chung Lin);郭薇雯(Wei-Wen Kuo);黃志揚(Chih-Yang Huang)*
Contributors:	中醫學院學士後中醫學系學士班中醫診斷學科
Keywords:	ginseng;Rg1;proliferate and migrate effect;Schwann cells;peripheral nerve regeneration
Date:	2009
Issue Date:	2009-08-20 18:04:40 (UTC+8)
Abstract:	The aim of the present study is to evaluate the proliferation- and migration-enhancing effects of ginseng and its component, ginsenoside (Rg1) on RSC96 Schwann cells. We investigated the molecular signaling pathways, which include: (1) survival signaling, IGFs-IGFIR-Akt-Bcl2 and proliferative signaling, cell cycle factors and mitogen-activated protein kinase (MAPK) pathways, (2) migrating and anti-scar signaling, FGF-2-uPA-MMPs.We treated RSC96 cells with different concentrations (100, 200, 300, 400, 500 µg ml-1) of ginseng and its constituent, Rg1 (5, 10, 15, 20, 25 µg ml-1). We observed a proliferative effect in a dose-dependent manner by PCNA western blotting assay, MTT assay, and wound healing test. Furthermore, we also found in the results of western blotting assay, ginseng and Rg1 enhance protein expression of IGF-I pathway regulators, cell cycle controlling proteins, and MAPK signaling pathways to promote the cell proliferation. In addition, ginseng and Rg1 also stimulated the FGF-2-uPA-MMP 9 migrating pathway to enhance the migration of RSC96 Schwann cells. Using MAPK chemical inhibitors, U0126, SB203580, and SP600125, the proliferative effects of ginseng and Rg1 on RSC96 cells were identified to be MAPK signaling-dependent. On the basis of the results, applying appropriate doses of ginseng and Rg1 with biomedical materials would be a potential approach for enhancing neuron regeneration. Copyright © 2009 John Wiley & Sons, Ltd.
Relation:	CELL BIOCHEMISTRY AND FUNCTION():~
Appears in Collections:	[School of Post-Baccalaureate Chinese Medicine] Journal articles

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