Potential ex vivo immunomodulatory effects of San-Huang-Xie-Xin-Tang and its component herbs on mice and humans

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Title:	Potential ex vivo immunomodulatory effects of San-Huang-Xie-Xin-Tang and its component herbs on mice and humans
Authors:	(Chia-Yang Li);侯鈺琪(Hou YuChi);李珮端(Pei Dawn Lee Chao);(Chi-Sheng Shia);(Ian C. Hsu);(Shih-Hua Fang)*
Contributors:	藥學院藥學系
Keywords:	San-Huang-Xie-Xin-Tang;Rheum officinale;Scutellaria baicalensis;Coptis chinesis;Peritoneal macrophage;Spleen cells;Peripheral blood mononuclear cells
Date:	2010-09
Issue Date:	2010-09-27 16:10:34 (UTC+8)
Abstract:	Aim of the study San-Huang-Xie-Xin-Tang (SHXXT), an important Chinese medicine formula, contains Rhei Rhizoma (RR), Scutellariae Radix (SR) and Coptidis Rhizoma (CR). RR and SR are abundant in anthraquinone and flavonoid polyphenols. Pharmacokinetic study of SHXXT indicated that glucuronides were the predominant forms of polyphenols in rats. Materials and methods As an extension of pharmacokinetic study, the serum metabolites of SHXXT, RR, SR and CR were prepared from rats and quantitated, then the immunomodulation effects were examined by culturing these serum metabolites with murine and human immune cells. Results The results indicated that the inhibitions on nitric oxide (NO) and cytokine production from mitogen-activated peritoneal macrophages by the serum metabolites of SHXXT, RR, SR and CR were through reducing the protein expression of inducible NO synthase (iNOS) and the IC50 were 0.8%, 1.5%, 3.0% and 0.8% of their blood concentrations, respectively. In addition, the serum metabolites of SHXXT, RR, SR and CR significantly decreased the ratios of interferon-gamma (IFN-γ) to interleukin (IL)-4 in mitogen-stimulated mice spleen cells and human peripheral blood mononuclear cells (PBMCs). Moreover, the serum metabolites of SHXXT and SR significantly arrested the mitogen-stimulated mice spleen cells at G2/M stage. Conclusions In conclusion, the serum metabolites of SHXXT and the component herbs exerted promising modulation activities on the immune functions and the cell cycle distribution of mice and human immune cells. We suggest that SHXXT is a promising remedy for immunomodulation through Th1/Th2 regulation.
Relation:	JOURNAL OF ETHNOPHARMACOLOGY 127(-):292-298
Appears in Collections:	[School of Pharmacy/Master Degree Program, Ph. D program] Journal articles

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