Proinflammatory cytokine gene polymorphisms among Hashimoto’s thyroditis patients

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Title:	Proinflammatory cytokine gene polymorphisms among Hashimoto’s thyroditis patients
Authors:	陳榮興;張淳堆(Chwen-Tzuei Chang);陳汶吉(Chen,Wen-Chi);蔡長海;蔡輔仁(Tsai,Fuu-Jen)*
Contributors:	中醫學院學士後中醫學系學士班中醫內科學科;中國附醫內科部新陳代謝科
Keywords:	Hashimoto's thyroiditis;polymorphism;proinflammatory cytokine
Date:	2006-11
Issue Date:	2009-08-20 18:04:16 (UTC+8)
Abstract:	Proinflammatory cytokines are involved in the pathogenesis of Hashimoto's thyroiditis (HT). To test whether certain specific proinflammatory cytokine gene polymorphisms could be genetic markers for an individual's susceptibility to HT, we investigated single-site polymorphisms of certain proinflammatory cytokine genes of interest for 107 HT sufferers and 163 controls, subsequent to preparing the necessary experimental genomic DNA from peripheral blood, using a polymerase chain reaction (PCR)-based restriction analysis. The polymorphisms we detected were as follows: 1) C/T and E1/E2 polymorphisms for the interleukin (IL)-1 gene at promoter (-511) and exon 5, respectively; 2) a variable number of tandem repeats (VNTRs) for the IL-1 receptor antagonist (IL-1Ra) gene at intron 2; 3) a C/G polymorphism for the IL-6 gene at promoter (-572); and 4) an A/G polymorphism for the tumor necrosis factor (TNF)- gene at promoter (-308). The data demonstrated an increased ratio of CG genotype and decreased ratios of CC and GG genotypes (chi-squared test; P = 0.025) for the IL-6 gene promoter for HT patients when compared with normal controls. The odds ratio (OR) for the CG genotype, as compared to the GG genotype, for HT patients was shown to be 4.065 (95% confidence interval (CI): 1.268-13.032). Comparison of the genotype analysis for the remaining gene polymorphisms and the allelic analysis for all of the screened gene polymorphisms, however, all revealed no statistically significant difference between the two study groups as regards frequency of genotype. In conclusion, we suggest that an IL-6 gene promoter (-572) C/G polymorphism could represent a potential candidate genetic marker to predict an individual's susceptibility to HT. J. Clin. Lab. Anal. 20:260-265, 2006. © 2006 Wiley-Liss, Inc.
Relation:	JOURNAL OF CLINICAL LABORATORY ANALYSIS20(20):260~265
Appears in Collections:	[School of Post-Baccalaureate Chinese Medicine] Journal articles

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