Metal ion mediated transfer and cleavage of diaminocarbene ligands

中國醫藥大學機構典藏 China Medical University Repository, Taiwan > 藥學院 > 藥學系暨碩博士班 > 期刊論文 > Item 310903500/31046

jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ir.cmu.edu.tw/ir/handle/310903500/31046

题名:	Metal ion mediated transfer and cleavage of diaminocarbene ligands
作者:	Ku, RZ;Huang, JC;Cho, JY;Kiang, FM;Reddy, KR;Chen, YC;Lee, KJ;Lee, JH;Lee, GH;Peng, SM;Liu, ST
贡献者:	藥學院藥學系;Natl Taiwan Univ, Dept Chem, Taipei 106, Taiwan;China Med Coll, Dept Pharm, Taichung, Taiwan
日期:	1999
上传时间:	2010-09-24 18:55:19 (UTC+8)
出版者:	AMER CHEMICAL SOC
摘要:	Protein C inhibitor (PCI) has been found in seminal plasma and is considered to protect intact surrounding cells and seminal plasma proteins from possible proteolytic damage. In the present study, we showed that although the antigenic levels of PCI in two seminal plasma samples from patients with infertility were normal or slightly elevated, their inhibitory activities toward urokinase plasminogen activator (uPA) and tissue-type plasminogen activator (tPA) were absent. In contrast, uPA and tPA proteolytic activities in these two samples were 20-60-fold higher than that from normal volunteers. A time-course analysis of PCI-uPA complex formation showed that >80% of the complex had been formed within 15 min in normal seminal plasma in the presence of heparin, compared with the total complex formed after 150 min incubation, whereas no response to heparin stimulation was observed in the assays with the two patient samples. Similarly, >90% of PCI-tPA complex was formed after 30 min of heparin stimulation in normal seminal plasma but no response was observed in the two patient samples. Kinetic assays of PCI inhibitory function in the presence of activated protein C (APC) showed that PCI inhibitory activity in the two patient samples was absent and not stimulated by heparin. Western blotting also showed that most of the intact PCI molecules, in normal samples, formed complexes with either uPA or tPA but there was no complex formed in one of the two patient samples and very little complex was observed in the other, suggesting that PCI in the two patient samples is inactive. These results suggest that the presence of functionally inactive PCI in seminal plasma may be associated with infertility.
關聯:	ORGANOMETALLICS 18(11):2145-2154
显示于类别:	[藥學系暨碩博士班] 期刊論文

文件中的档案:

没有与此文件相关的档案.

在CMUR中所有的数据项都受到原著作权保护.

TAIR相关文章

数据加载中.....