High-performance liquid chromatography determination of methionine adenosyltransferase activity using catechol-O-methyltransferase-coupled fluorometric detection

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Title:	High-performance liquid chromatography determination of methionine adenosyltransferase activity using catechol-O-methyltransferase-coupled fluorometric detection
Authors:	Wang, SH;Kuo, SC;Chen, SC
Contributors:	藥學院藥化所;China Med Coll, Grad Inst Pharmaceut Chem, Taichung, Taiwan
Date:	2003
Issue Date:	2010-09-24 15:08:50 (UTC+8)
Publisher:	ACADEMIC PRESS INC ELSEVIER SCIENCE
Abstract:	Technetium-99m Tetrofsomin (Tc-TF) has been shown to be useful in identifying several types of tumors, such as breast, lung, and thyroid cancers. There was no report in the literature for Tc-TF uptake in hepatocellular carcinoma (HCC). The aim of this study was to evaluate the usefulness of Tc-TF liver imaging to detect HCC and investigate the relationship between Tc-TF liver imaging findings and P-glycoprotein (Pup) and multidrug resistance associated protein (MRP) expression. Before any therapy, 22 patients with HCC were enrolled in this study. Tc-TF liver images were performed 10 minutes after intravenous injection of 20mCi Tc-TF. All patients had liver biopsy or surgery within I week after Tc-TF liver imaging. Immunohistochemical study of the biopsy or resected HCC specimens was performed using anti-human Pup and MRP antibodies. Twenty of the 22 (90.9%) patients showed negative Tc-TF liver imaging results without significant Tc-TF uptake in HCC, whereas only the remaining 2 (9.1%) patients showed positive Tc-TF liver imaging results with significant Tc-TF uptake in HCC. Positive Pgp expression was observed in 13 of 20 patients with negative Tc-TF liver imaging results, whereas positive MRP expression was observed in 6 of the remaining 7 patients with negative both Tc-TF liver imaging results and Pgp expression. However, negative Pgp expression but positive MRP expression was observed in all of the remaining 2 patients with positive Tc-TF liver imaging results. The correlation between Tc-TF liver imaging findings and Pgp expression was significant and better than between Tc-TF liver imaging findings and MRP expression. Pgp or MRP expression in HCC may induce no significant Tc-TF uptake in HCC resulting in negative Tc-TF liver imaging findings. Therefore, Tc-TF liver imaging is potential to be a non-invasive method to predict Pup or MRP expression in HCC. However, further studies with a larger series of patients and longer follow-up time are necessary to confirm our findings. (C) 2003 Elsevier Inc. All rights reserved.
Relation:	ANALYTICAL BIOCHEMISTRY 319(1):13-20
Appears in Collections:	[Graduate Institute of Pharmaceutical Chemistry] Journal articles

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