HPLC determination of 2-phenyl-4-quinolone in rabbit serum

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题名:	HPLC determination of 2-phenyl-4-quinolone in rabbit serum
作者:	Huang, TH;Cheng, HH;Tsai, SY;Chao, PDL;Kuo, SC
贡献者:	藥學院藥化所;China Med Coll, Grad Inst Pharmaceut Chem, Taichung, Taiwan;China Med Coll, Sch Pharm, Taichung, Taiwan
日期:	2001
上传时间:	2010-09-24 15:08:13 (UTC+8)
出版者:	MARCEL DEKKER INC
摘要:	The cytoskeleton plays important roles in eel function and is therefore implicated in the pathogenesis of many human liver diseases, including malignant tumors. The stability of cytokeratin proteins during tumor transformation in human hepatocellular carcinoma has been studied with a molecular approach previously. The results demonstrate that the cytokeratin is modulated in human hepatocellular carcinoma. Besides this, three low molecular weight cytokeratin molecules (named HCC CK) are found. This indicates that these HCC CKs have undergone modulation from the human hepatocyte cytokeratin Is. We also checked the cytokeratin profile of the human hepatoma cell line PLC/PRF/5 with the same methods to ensure the HCC CK molecules are produced by modulation but not protein degradation. The stability of cytokeratin molecules was studied by a different approach. The cytokeratin compositions of human liver cells (Chang cell line) were analysed under the effects of microtubule-disrupting drug (colchicine) by SDS-PAGE, Western blot, immunoprecipitation using a commercially available monoclonal anti-cytokeratin 18 antibody and immunofluorescent staining, Within 1 h of treatment, the microtubule began to collapse and the filamentous structure was shortening. The microtubule had almost collapsed and became fragmented to form a lattice-like network after 24 h of treatment. The cytokeratin was modulated after long-term (24 h) treatment of colchicine, and the molecular weight became 14 kD and the antigenicity was lost. The stability of cytokeratin molecules was related to the intact microtubule network, after disruption of the microtubule the cytokeratin would be modulated. The intact microtubule network was a stabilizing factor of cytokeratin 18 in human liver cells. (C) 2001 Elsevier Science Ltd, All rights reserved.
關聯:	JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 24(1):79-85
显示于类别:	[藥物化學研究所] 期刊論文

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