Effects of aspirin on the in vitro and in vivo acetylation of 2-aminofluorene in Sprague-Dawley rats

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Title:	Effects of aspirin on the in vitro and in vivo acetylation of 2-aminofluorene in Sprague-Dawley rats
Authors:	Lin, SS;Yu, CS;Wu, JY;Tyan, YS;Hsia, TC;Lin, WC;Chung, JG
Contributors:	醫學院醫學系微生物學科;China Med Univ, Sch Med, Dept Microbiol, Taichung 404, Taiwan;Chungtai Inst Hlth Sci & Technol, Dept Radiol Technol, Taichung, Taiwan;China Med Univ, Ctr Gen Educ, Taichung 404, Taiwan;China Med Univ, Dept Pharmacol, Taichung 404, Taiwan;China Med Univ, Dept Microbiol, Taichung 404, Taiwan;Armed Forces Taichung Gen Hosp, Dept Chest Surg, Taichung, Taiwan;Chung Shan Med Univ, Dept Radiol, Taichung, Taiwan;China Med Univ Hosp, Dept Internal Med, Taichung, Taiwan
Date:	2005
Issue Date:	2010-09-24 15:03:13 (UTC+8)
Publisher:	INT INST ANTICANCER RESEARCH
Abstract:	Objective Steroid 11 beta-hydroxylase (CYP11B1) deficiency, an autosomal recessive inherited disease, accounts for 5-8% of congenital adrenal hyperplasia (CAH). It is mainly caused by mutations of nucleotide substitutions in the coding region. Patients and Methods The study reports on a 9-year-old Chinese boy who presented with a bone age of 16 years, an enlarged penis, an accelerated growth rate since early childhood and hypertension (160-170/100-110 mmHg) for 3 years. Because it shares 95% sequence homology with aldosterone synthetase (CYP11B2), we developed gene-specific primers for differential PCR amplification of the CYP11B1 gene. The secondary PCR products of nine exons of the CYP11B1 gene were then subjected to single-strand conformation polymorphism (SSCP) analysis and DNA sequencing. The serum hormone levels were also determined. Results We found that the boy diagnosed with CAH due to 11 beta-hydroxylase deficiency carried mutations of A306V (GCC- > GTC) and T318P (ACG- > CCG) in two respective chromosomes. The hormone assay showed that the 11-deoxycortisol level was higher (667 nmol/l) than normal and was further increased after ACTH stimulation (1206 nmol/l). Conclusions These two mutations have not previously been described in the CYP11B1 gene. The discovery of these two novel mutations increases our knowledge of CAH caused by 11 beta-hydroxylase deficiency.
Relation:	IN VIVO 19(2):475-481
Appears in Collections:	[School of Medicine] Journal articles

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