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    题名: Differential gene expression of scopolamine-treated rat hippocampus-application of cDNA microarray technology
    作者: Hsieh, MT;Hsieh, CL;Lin, LW;Wu, CR;Huang, GS
    贡献者: 藥學院中藥所;China Med Coll Hosp, Inst Chinese Pharmaceut Sci, Taichung 404, Taiwan
    日期: 2003
    上传时间: 2010-09-24 15:01:54 (UTC+8)
    出版者: PERGAMON-ELSEVIER SCIENCE LTD
    摘要: N-Acetyltransferases (NATs) plays an important role in the first step of arylamine compounds metabolism. Polymorphic NAT is coded for rapid or slow acetylatoion phenotypes, which are recognized to affect cancer risk related to environmental exposure. Aloe-emodin has been shown to exit anticancer activity. The purpose of this study is to examine whether or not aloe-emodin could affect arylamine N-acetyltransferase (NAT) activity and gene expression (NAT mRNA) and DNA-2-aminofluorene (DNA-AF) adduct formation in mouse leukemia cells (L 1210). By using high performance liquid chromatography, N-acetylation and non-N-acetylation of AF were determined and quantitated. By using reverse transcriptase-polymerase chain reaction (RT-PCR) and PCR, NAT mRNA was determined and quantitated. Aloe-emodin displayed a dose-dependent inhibition to cytosolic NAT activity and intact mice leukemia cells. Time-course experiments indicated that N-acetylation of AF measured from intact mice leukemia cells were inhibited by aloe-emodin for up to 24 h. Using standard steady-state kinetic analysis, it was demonstrated that aloe-emodin was a possible uncompetitive inhibitor to NAT activity in cytosols. The DNA-AF adduct formation in mouse leukemia cells were inhibited by aloe-emodin. The NAT1 mRNA in mouse leukemia cells were also inhibited by aloe-emodin. This report is the first demonstration which showed aloe-emodin affect mice leukemia cells NAT activity, gene expression (NAT] mRNA) and DNA-AF on adduct formation. (C) 2003 Elsevier Science Ltd. All rights reserved.
    關聯: LIFE SCIENCES 73(8):1007-1016
    显示于类别:[中國藥學研究所(已停用)] 期刊論文

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