Activation of activator protein 1 in cells exposed to electrical stimulation of various parameters used in electro-acupuncture therapy.

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Title:	Activation of activator protein 1 in cells exposed to electrical stimulation of various parameters used in electro-acupuncture therapy.
Authors:	許昇峰(Hsu,Sheng-Feng);林昭庚;劉旭然(Hsu-Jan Liu);賴羿如(I-Lu Lai);吳世祿(Shih-Lu Wu);項千芸(Chien-Yun Hsiang);侯庭鏞(Tin-Yun Ho)*
Contributors:	中醫學院針灸研究所;中國附醫中醫針灸科
Keywords:	Activator Protein-1;Electrical Stimulation;Electroacupuncture
Date:	2003-12
Issue Date:	2009-08-20 17:59:21 (UTC+8)
Abstract:	The aim of this study was to investigate the effect of electrical stimulation on activator protein-1 (AP-1) in recombinant liver cells. In order to elucidate the molecular effects of electrical stimulation on cells, AP-1 expression was detected by a luciferase assay. The parameters used were taken from clinical electroacupuncture (EA) therapy as follows: biphasic rectangular symmetrical pulses (frequency: 2, 10 and 100 Hz; pulse width: 50, 80, 130 and 250 μsec; intensity: 1, 2, 5 and 10 mA; time: 5, 10, 20 and 30 minutes). S (10% fetal bovine serum in medium), SF (serum free medium) and 12-O-tetradecanoylphorbol-13-acetate (TPA) treatment represented the experimental, negative and positive groups, respectively. We found that electrical stimulation with 10 Hz, a pulse width of 130 μsec, and a duration of 30 minutes gave a significant increase in AP-1 activity. In contrast, the intensity of the stimulation had no significant effect on AP-1 activity. In conclusion, our data suggest that electrical stimulation with an appropriate frequency, duration and pulse width could cause an increase in AP-1 activity in cells.
Relation:	AMERICAN JOURNAL OF CHINESE MEDICINE 31(6):967~975
Appears in Collections:	[Graduate Institute of Acupuncture Science] Journal articles

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