Abscisic acid promotes development of somatic embryos on converted somatic embryos of Corydalis yanhusuo (Fumariaceae)

CMUR > College of Pharmacy > Graduate Institute of Chinese Pharmaceutical Science > Journal articles > Item 310903500/30783

Please use this identifier to cite or link to this item: http://ir.cmu.edu.tw/ir/handle/310903500/30783

Title:	Abscisic acid promotes development of somatic embryos on converted somatic embryos of Corydalis yanhusuo (Fumariaceae)
Authors:	Kuo, CL;Sagare, AP;Lo, SF;Lee, CY;Chen, CC;Tsay, HS
Contributors:	藥學院中藥所;Taiwan Agr Res Inst, Dept Agron, Taichung 41301, Taiwan;Chung Hwa Coll Med Technol, Tainan 71705, Taiwan;China Med Coll, Inst Chinese Pharmaceut Sci, Taichung 40421, Taiwan
Date:	2002
Issue Date:	2010-09-24 15:01:40 (UTC+8)
Publisher:	URBAN & FISCHER VERLAG
Abstract:	In this study, paclitaxel was used to determine inhibition of arylamine X-acetyltransferase (NAT) activity. gene expresssion and 2-aminofluorene-DNA adduct formation in a human lung tumor cell line (A549). The activity of NAT was measured by HPLC assaying for the amounts of N-acetyl-2-aminofluorene (2-AAF) and remaining 2-aminofluorene (2-AF). Human lung tumor cell cytosols and intact cells were used for examining NAT activity and carcinogen-DNA adduct formation. The results demonstrated that NAT activity, gene expression (NAT1 mRNA) and 2-AF-DNA adduct formation in human lung tumor cells were inhibited and decreased by paclitaxel in a dose-dependent manner. The effects of paclitaxel on the values of the apparent K-m and V-max of NAT from human lung tumor cells were also determined in both examined systems. The result also indicated that paclitaxel decreased the apparent values of K-m and V-max from human lung tumor cells in both cytosol and intact cells. Thus, paclitaxel is an uncompetitive inhibitor to NAT enzyme. (C) 2002 Elsevier Science Ltd. All rights reserved.
Relation:	JOURNAL OF PLANT PHYSIOLOGY 159(4):423-427
Appears in Collections:	[Graduate Institute of Chinese Pharmaceutical Science] Journal articles

Files in This Item:

There are no files associated with this item.

Loading...