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| 題名: | Disturbance of circadian gene expression in endometrial cancer: Detection by real-time quantitative RT-PCR |
| 作者: | Shih, HC;Choo, KB;Chang, TJ;Yang, MY;Shih, MC;Yeh, KT;Liu, TC;Lin, SF;Chang, JG |
| 貢獻者: | 附設醫院醫研部分子醫學中心;China Med Univ Hosp, Dept Mol Med, Taichung, Taiwan;Natl Chung Hsing Univ, Coll Vet Med, Taichung 40227, Taiwan;Taipei Vet Gen Hosp, Dept Med Res & Educ, Taipei, Taiwan;Kaohsiung Med Univ, Dept Hematol Oncol, Kaohsiung, Taiwan;Changhua Christian Hosp, Dept Pathol, Changhua, Taiwan |
| 日期: | 2005 |
| 上傳時間: | 2010-09-24 15:01:36 (UTC+8) |
| 出版者: | PROFESSOR D A SPANDIDOS |
| 摘要: | NF-E2 is a transcription activator for the regulation of a number of erythroid- and megakaryocytic lineage-specific genes. Here we present evidence that the large subunit of mammalian NF-E2, p45, is sumoylated in vivo in human erythroid K562 cells and in mouse fetal liver. By in vitro sumoylation reaction and DNA transfection experiments, we show that the sumoylation occurs at lysine 368 (K368) of human p45/NF-E2. Furthermore, p45 sumoylation enhances the transactivation capability of NF-E2, and this is accompanied by an increase of the NF-E2 DNA binding affinity. More interestingly, we have found that in K562 cells, the beta-globin gene loci in the euchromatin regions are predominantly colocalized with the nuclear bodies promyelocytic leukemia protein (PML) oncogenic domains that are enriched with the PML, SUMO-1, RNA polymerase H, and sumoylatable p45/NF-E2. Chromatin immunoprecipitation assays further showed that the intact sumoylation site of p45/ NF-E2 is required for its binding to the DNase I-hypersensitive sites of the beta-globin locus control region. Finally, we demonstrated by stable transfection assay that only the wild-type p45, but not its mutant form p45 (K368R), could efficiently rescue beta-globin gene expression in the p45-null, erythroid cell line CB3. These data together point to a model of mammalian P-like globin gene activation by sumoylated p45/NF-E2 in erythroid cells. |
| 關聯: | ONCOLOGY REPORTS 14(6):1533-1538 |
| 顯示於類別: | [台中附設醫院] 期刊論文
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