No mutation of G209A in the alpha-synuclein gene in sporadic Parkinson's disease among Taiwan Chinese

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Title:	No mutation of G209A in the alpha-synuclein gene in sporadic Parkinson's disease among Taiwan Chinese
Authors:	Hu, CJ;Sung, SM;Liu, HC;Chang, JG
Contributors:	附設醫院醫研部分子醫學中心;China Med Coll Hosp, Div Mol Med, Dept Med Res, Taichung, Taiwan;Taipei Municipal Jen Ai Hosp, Dept Neurol, Taipei, Taiwan;Taipei City Psychiat Ctr, Taipei, Taiwan
Date:	1999
Issue Date:	2010-09-24 15:01:30 (UTC+8)
Publisher:	KARGER
Abstract:	A modified high-performance chromatographic method using UV detection was developed for determination of tramadol concentration in human plasma. Plasma samples were extracted with ethyl acetate in a one-step liquid-liquid extraction (recovery 88.5+/-2.1%). Analysis of the extract was performed on a reversed-phase LiChrospher 60 RP-select B column with a particle size of 5 mu m. The mobile phase consisted of 0.05 M KH2PO4 aqueous solution (pH 3.5) and acetonitrile in a ratio of 90:10 (v/v). Metoprolol was used as the internal standard and UV detection at 225 nm was employed. Accuracy of the assay in the concentration range examined was from 1.3 to 11.9% for the intra-day run and from 1.4 to 8.1% for the inter-day run. The precision of this method varied from 1.2 to 8.7%. The reproducibility of the method was determined to be from 0.8 to 7.2% over the six-day period. A limit of detection was 9 ng/ml at a signal-to-noise ratio of 3. This validated method was then applied to the determination of tramadol concentrations in healthy volunteers after oral administration of 100 mg of tramadol in capsules of Painlax(R) and Tramal(R). (C) 1999 Elsevier Science B.V. All rights reserved.
Relation:	EUROPEAN NEUROLOGY 41(2):85-87
Appears in Collections:	[China Medical University Hospital] Jurnal articles

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