Uncaria rhynchophylla and rhynchophylline inhibit c-Jun N-terminal kinase phosphorylation and nuclear factor-κB activity in kainic acid-treated rats.

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Title:	Uncaria rhynchophylla and rhynchophylline inhibit c-Jun N-terminal kinase phosphorylation and nuclear factor-κB activity in kainic acid-treated rats.
Authors:	謝慶良(Ching-Liang Hsieh);侯庭鏞(Tin-Yun Ho);蘇珊玉(Shan-Yu Su);羅婉瑜(Wan-Yu Lo);劉崇祥(Chung-Hsiang Liu);唐娜櫻(Nou-Ying Tang)*
Contributors:	中醫學院針灸研究所;中國附醫中醫內科
Keywords:	Uncaria rhynchophylla;Rhynchophyllin;Nuclear Factor-κB;Mitogen-Activated Protein Kinase;c-Jun N-Terminal Kinase;Kainic Acid;Epilepsy
Date:	2009-04
Issue Date:	2009-08-20 17:59:20 (UTC+8)
Abstract:	Our previous studies have shown that Uncaria rhynchophylla (UR) can reduce epileptic seizures. We hypothesized that UR and its major component rhynchophylline (RH), reduce epileptic seizures in rats treated with kainic acid (KA) by inhibiting nuclear factor-κB (NF-κB) and activator-protein-1 (AP-1) activity, and by eliminating superoxide anions. Therefore, the level of superoxide anions and the DNA binding activities of NF-κB and AP-1 were measured. Sprague-Dawley (SD) rats were pre-treated with UR (1.0 g/kg, i.p.), RH (0.25 mg/kg, i.p.), or valproic acid (VA, 250 mg/kg, i.p.) for 3 days and then KA was administered intra-peritoneal (i.p.). The results indicated that UR, RH, and VA can reduce epileptic seizures and the level of superoxide anions in the blood. Furthermore, KA was demonstrated to induce the DNA binding activities of NF-κB and AP-1. However, these inductions were inhibited by pre-treatment with UR, RH, or VA for 3 days. Moreover, UR and RH were shown to be involved in the suppression of c-Jun N-terminal kinase (JNK) phosphorylation. This study suggested that UR and RH have antiepileptic effects in KA-induced seizures and are associated with the regulation of the innate immune system via a reduction in the level of superoxide anions, JNK phosphorylation, and NF-κB activation.
Relation:	AMERICAN JOURNAL OF CHINESE MEDICINE37(2):351~360
Appears in Collections:	[Graduate Institute of Acupuncture Science] Journal articles

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