Asymmetrical synthesis of L-homophenylalanine using engineered Escherichia coli aspartate aminotransferase

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Title:	Asymmetrical synthesis of L-homophenylalanine using engineered Escherichia coli aspartate aminotransferase
Authors:	Lo, HH;Hsu, SK;Lin, WD;Chan, NL;Hsu, WH
Contributors:	附設醫院醫研部;Natl Chung Hsing Univ, Inst Mol Biol, Taichung 402, Taiwan;Natl Chung Hsing Univ, Inst Biochem, Taichung 402, Taiwan;Chungtai Inst Hlth Sci & Technol, Dept Med Technol, Taichung 406, Taiwan;Chungtai Inst Hlth Sci & Technol, Dept Dent Technol, Taichung 406, Taiwan;China Med Univ Hosp, Dept Med Res, Taichung 404, Taiwan
Date:	2005
Issue Date:	2010-09-24 15:01:04 (UTC+8)
Publisher:	AMER CHEMICAL SOC
Abstract:	It is well documented that arylamine carcinogens are N-acetylated by cytosolic N-acetyltransferase (NAT) enzyme. NAT plays an important role in the metabolizing of those arylamine compounds. 2-Aminofluorene (AF) is an arylamine carcinogen which has been demonstrated to induce carcinogenesis in laboratory animals. Our previous study has shown that a human promyelocytic leukemia cell line, HL-60, displays NAT activity. The purpose of the present study was to determine whether or not wogonin could affect the N-acetylation of AF in HL-60. N-acetylated and non-N-acetylated AF were determined by using high performance liquid chromatography. Wogonin displayed a dose-dependent inhibition of NAT activity in cytosols and intact cells. Wogonin also decreased AF-DNA adduct formation in these cells. The effects of wogonin on the NA T enzymes levels were also examined by Western blotting and flow cytometry and the changes of NAT gene expression were examined by polymerase chain reaction (PCR) and cDNA microarray. The results demonstrated that wogonin inhibited NAT1 mRNA gene expression and the level of NAT enzyme in HL-60 cells. This is the first demonstration that wogonin affects human leukemia cells' NAT activity in vitro.
Relation:	BIOTECHNOLOGY PROGRESS 21(2):411-415
Appears in Collections:	[China Medical University Hospital] Jurnal articles

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