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| 題名: | Deregulated expression of the PER1, PER2 and PER3 genes in breast cancers |
| 作者: | Chen, ST;Choo, KB;Hou, MF;Yeh, KT;Kuo, SJ;Chang, JG |
| 貢獻者: | 附設醫院醫研部;China Med Univ Hosp, Dept Mol Med, Taichung, Taiwan;Changhua Christian Hosp, Dept Surg, Changhua, Taiwan;Changhua Christian Hosp, Dept Pathol, Changhua, Taiwan;Taipei Vet Gen Hosp, Dept Med Res Educ, Taipei, Taiwan;Kaohsiung Med Univ, Dept Surg, Kaohsiung, Taiwan;Taipei Inst Pathol, Taipei, Taiwan |
| 日期: | 2005 |
| 上傳時間: | 2010-09-24 15:00:44 (UTC+8) |
| 出版者: | OXFORD UNIV PRESS |
| 摘要: | In Xanthomonas campestris, the causative agent of black rot in crucifers, the endoglucanase level is greatly decreased in the mutant deficient in Clip, a homologue of cyclic AMP receptor protein (CRP). It is established that Clp has the same DNA binding specificity as CRP at positions 5, 6, and 7 (GTG motif) of the DNA half site. In this study, the engA transcription initiation site was determined by the 5' RACE method, and two consensus Clp-binding sites, site I and site II centered at -69.5 and -42.5, respectively, were located. Transcriptional fusion assays indicated that Clp greatly activates engA transcription. Site-directed mutagenesis indicated that position 5 of GTG motif in site II is essential for both DNA-protein complex formation in electrophoretic mobility shift assays and engA transcription in vivo. In addition, mutation at position 5 of site I drastically reduces the promoter activity, indicating that binding of Clp to site I exerts a synergistic effect on the transcription activation by site II. engA appears to be the first X. campestris gene known to be activated by Clp via a direct binding to the promoter. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V.. All rights reserved. |
| 關聯: | CARCINOGENESIS 26(7):1241-1246 |
| 顯示於類別: | [台中附設醫院] 期刊論文
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