Reversal caused by n-butylidenephthalide from the deficits of inhibitory avoidance performance in rats

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Title:	Reversal caused by n-butylidenephthalide from the deficits of inhibitory avoidance performance in rats
Authors:	Hsieh, MT;Wu, CR;Lin, LW;Hsieh, CC;Tsai, CH
Contributors:	藥學院中藥所;China Med Coll, Inst Chinese Pharmaceut Sci, Taichung, Taiwan;China Med Coll, Inst Med Sci, Taichung, Taiwan
Date:	2001
Issue Date:	2010-09-24 15:00:42 (UTC+8)
Publisher:	GEORG THIEME VERLAG KG
Abstract:	A simple protocol for in vitro mass propagation of Gentiana davidii var. formosana (Gentianaceae) has been developed. Multiple shoot development was achieved by culturing the stem node explants on Murashige and Skoog (MS) medium supplemented with 4.44 muM N-6-benzyladenine (BA). The shoots were multiplied by subculturing on MS medium supplemented with 1.07-10,74 muM alpha -naphthaleneacetic acid (NAA) and 8.88 muM BA. Shoots were Footed on MS basal medium supplemented with various auxins. Shoots Footed on growth regulator-free medium were transferred to peat moss:vermiculite mixture and acclimatized in the growth chamber. The contents of gentiopicroside and swertiamarin, the two important secoiridoid glucosides, in different plant material were determined by high performance liquid chromatography (HPLC). The analysis revealed that the content of gentiopicroside and swertiamarin in the aerial and underground parts of G.davidii var, formosana was higher than the marketed crude drug (underground parts of G.scabra) and varied with the age of the plant.
Relation:	PLANTA MEDICA 67(1):38-42
Appears in Collections:	[Graduate Institute of Chinese Pharmaceutical Science] Journal articles

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