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請使用永久網址來引用或連結此文件:
http://ir.cmu.edu.tw/ir/handle/310903500/30711
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| 題名: | Analysis of three lupane type triterpenoids in Helicteres angustifolia by high-performance liquid chromatography |
| 作者: | Chang, YS;Ku, YR;Lin, JH;Lu, KL;Ho, LK |
| 貢獻者: | 藥學院中藥所;China Med Coll, Inst Chinese Pharmaceut Sci, Taichung 404, Taiwan;Natl Labs Foods & Drugs, Dept Hlth, Taipei 115, Taiwan;Chung Hwa Inst Technol, Tainan 717, Taiwan;Natl Yang Ming Univ, Dept & Inst Pharmacol, Taipei 112, Taiwan |
| 日期: | 2001 |
| 上傳時間: | 2010-09-24 15:00:25 (UTC+8) |
| 出版者: | PERGAMON-ELSEVIER SCIENCE LTD |
| 摘要: | 17-Hydroxyprogesterone (17OHP) is the most important plasma parameter for diagnosing and monitoring congenital adrenal hyperplasia (CAH) caused by 21-hydroxylase deficiency. A rapid, simple, and specific method based on microbore high-performance liquid chromatography/electrospray ionization tandem mass spectrometry (mu -HPLC/ESI-MS/MS) was developed to determine the presence of 17OHP on dried filter-paper blood samples from patients with CAH caused by 21-hydroxylase deficiency. 17OHP from dried blood spots formed by the action of Girard reagent P (Girl?) turned out to be a water-soluble hydrazone complex. Derivatization with Girl? led to higher ESI sensitivity for 17OHP. The LC/MS/MS detection of GirP-derivatized 17OHP (GirP-17OHP) was rapid (<3 min). The method is repeatable and reproducible, with CVs <7% and 12%, respectively. This new method was used for direct determination of 17OHP in dried blood specimens obtained from abnormal children and infants of various ages with a detection limit of 10 ng/mL (similar to 12 muL blood). The method described allows for rapid and reliable measurements of 17OHP in dried blood specimens from patients affected by CAH. Copyright (C) 2001 John Wiley & Sons, Ltd. |
| 關聯: | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 26(5月6日):849-855 |
| 顯示於類別: | [中國藥學研究所(已停用)] 期刊論文
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