In vitro propagation of Limonium wrightii (Hance) Ktze. (Plumbaginaceae), an ethnomedicinal plant, from shoot-tip, leaf- and inflorescence-node explants

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题名:	In vitro propagation of Limonium wrightii (Hance) Ktze. (Plumbaginaceae), an ethnomedicinal plant, from shoot-tip, leaf- and inflorescence-node explants
作者:	Huang, CL;Hsieh, MT;Hsieh, WC;Sagare, AP;Tsay, HS
贡献者:	藥學院中藥所;China Med Coll, Inst Chinese Pharmaceut Sci, Taichung 41421, Taiwan;Taiwan Agr Res Inst, Dept Agron, Taichung 41301, Taiwan
日期:	2000
上传时间:	2010-09-24 15:00:13 (UTC+8)
出版者:	SOC IN VITRO BIOLOGY
摘要:	The role of epidermal growth factor receptors (EGF-R) in osteogenic cell differentiation was investigated using preosteoblastic MC3T3-E1 (MC3T3) cells and osteoblast-like ROS 17/2.8 (ROS) cells. When cultured in the presence of P-glycerophosphate (GP) and ascorbic acid (AA), MC3T3 cells underwent spontaneous differentiation into osteoblasts which was confirmed as they expressed osteoblast markers such as alkaline phosphatase (ALP), bone sialoprotein (BSP) and osteocalcin (OC). Interestingly, the number of EGF-binding sites decreased during their differentiation into osteoblasts, and the osteogenic protein-1 (OP-1) treatment, which accelerated their differentiation, lowered the number of EGF-binding sites even further. On the other hand, ROS cells with high expression levels of osteoblast markers and no EGF-R, after being transfected with human EGF-R cDNA (EROS cells), expressed numerous EGF-binding sites as well as EGF-R mRNA and protein; in the process, they ceased to express osteoblast markers, indicating their dedifferentiation into osteoprogenitor cells. Both MC3T3 and EROS cells showed increased cell growth in response to EGF, whereas ROS cells did not. These results imply that the EGF/EGF-R system in osteogenic cells has a crucial function in osteoblast phenotype suppression and osteogenic cell proliferation.
關聯:	IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT 36(3):220-224
显示于类别:	[中國藥學研究所(已停用)] 期刊論文

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