Autosomal dominant avascular necrosis of femoral head in two Taiwanese pedigrees and linkage to chromosome 12q13

中國醫藥大學機構典藏 China Medical University Repository, Taiwan > 台中附設醫院 > 期刊論文 > Item 310903500/30681

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题名:	Autosomal dominant avascular necrosis of femoral head in two Taiwanese pedigrees and linkage to chromosome 12q13
作者:	Chen, WM;Liu, YF;Lin, MW;Chen, IC;Lin, PY;Lin, GL;Jou, YS;Lin, YT;Fann, CSJ;Wu, JY;Hsiao, KJ;Tsai, SF
贡献者:	附設醫院醫研部;Natl Hlth Res Inst, Div Mol & Genom Med, Taipei 115, Taiwan;Acad Sinica, Inst Biomed Sci, Taipei, Taiwan;Taipei Vet Gen Hosp, Dept Orthopaed & Traumatol, Taipei, Taiwan;Taipei Vet Gen Hosp, Dept Med Res & Educ, Taipei, Taiwan;Taipei Vet Gen Hosp, Dept Ophthalmol, Taipei, Taiwan;Natl Yang Ming Univ, Sch Med, Fac Med, Taipei 112, Taiwan;Natl Yang Ming Univ, Genet Inst, Taipei 112, Taiwan;Natl Yang Ming Univ, Genome Res Ctr, Taipei 112, Taiwan;Chain An MIS Med Ctr, Chungli, Taiwan;China Med Univ Hosp, Dept Med Res, Taichung, Taiwan
日期:	2004
上传时间:	2010-09-24 14:59:56 (UTC+8)
出版者:	UNIV CHICAGO PRESS
摘要:	AIM: Five representative waterborne pathogens were used to illustrate the relationship between chitosan's antibacterial activity and the surface characteristics of the bacterial cell wall. METHODS: Chitosan was prepared with averaged 75 % or 95 % deacetylated degree to examine its antibacterial activity against waterborne pathogens. Fresh microbial inoculants for the antibacterial assessment were prepared on nutrient agar at 37 degreesC for 24 h. The evaluation items of antibacterial mechanism included hydrophilicity and negative charge analysis of cell surface, and adsorptive characteristics of chitosan to bacterial cell. All the experiments were applied in triplicate tests at least. RESULTS: Although cell wall hydrophilicity was similar among Gram-negative bacteria, the distribution of negative charge on their cell surfaces was quite different. More negatively charged cell surfaces had a greater interaction with chitosan, a phenomenon further confirmed by transmission electron micrography (TEM). CONCLUSION: Results showed the hydrophilicity in Gram-negative bacteria was much higher than in Gram-positive ones. The correlation coefficient 0.988 between the amount of absorbed chitosan and its inhibition efficiency indicated a close relationship.
關聯:	AMERICAN JOURNAL OF HUMAN GENETICS 75(2):310-317
显示于类别:	[台中附設醫院] 期刊論文

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