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http://ir.cmu.edu.tw/ir/handle/310903500/30656
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| 題名: | Mutational, epigenetic and expressional analyses of caveolin-1 gene in breast cancers |
| 作者: | Chen, ST;Lin, SY;Yeh, KT;Ku, SJ;Chan, WL;Chu, YP;Chang, JG |
| 貢獻者: | 附設醫院醫研部;China Med Univ, Dept Mol Med, Taichung, Taiwan;Changhua Christian Hosp, Dept Surg, Changhua, Taiwan;Taipei Med Univ, Dept Internal Med, Taipei, Taiwan;Natl Chung Shing Univ, Dept Comp Sci, Taichung, Taiwan;Taipei Inst Pathol, Taipei, Taiwan |
| 日期: | 2004 |
| 上傳時間: | 2010-09-24 14:59:29 (UTC+8) |
| 出版者: | PROFESSOR D A SPANDIDOS |
| 摘要: | Severe acute respiratory syndrome (SARS) has been globally reported. A novel coronavirus (CoV), SARS-CoV, was identified as the etiological agent of the disease. SARS-CoV 3C-like protease (3CL(pro)) mediates the proteolytic processing of replicase polypeptides la and lab into functional proteins, playing an important role in viral replication. In this study, we demonstrated the expression of the SARS-CoV 3CL(pro) in Escheichia coli and Vero cells, and then characterized the in vitro trans-cleavage and the cell-based cis-cleavage by the 3CL(pro). Mutational analysis of the 3CL(pro) demonstrated the importance of His41, Cys145, and Glu166 in the substrate-binding subsite SI for keeping the proteolytic activity. In addition, alanine substitution of the cleavage substrates indicated that Gln-(p1) in the substrates mainly determined the cleavage efficiency. Therefore, this stud), not only established the quantifiable and reliable assay for the in vitro and cell-based measurement of the 3CL(pro) activity, but also characterized the molecular interaction of the SARS-CoV 3CL(pro) with the substrates. The results will be useful for the rational development of the anti-SARS drugs. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. |
| 關聯: | INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE 14(4):577-582 |
| 顯示於類別: | [台中附設醫院] 期刊論文
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