Functionally inactive protein C inhibitor in seminal plasma may be associated with infertility

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Title:	Functionally inactive protein C inhibitor in seminal plasma may be associated with infertility
Authors:	He, SP;Lin, YL;Liu, YX
Contributors:	醫學院醫學系寄生蟲學科;Univ Birmingham, Queen Elizabeth Hosp, Sch Med, Dept Med, Birmingham B15 2TH, W Midlands, England;China Med Coll, Sch Med, Dept Parasitol, Taichun 40421, Taiwan;Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100080, Peoples R China
Date:	1999
Issue Date:	2010-09-24 14:59:02 (UTC+8)
Publisher:	OXFORD UNIV PRESS
Abstract:	Benzophenone is an ultraviolet (UV)-absorbing agent that has been used in industry and medicine for more than 30 years. Consumers of cosmetics and sunscreens containing UV-absorbers are exposed to benzophenones on a daily basis, owing to the widespread use of these compounds. However, the efficacy of these compounds as scavengers of oxidative stress is still not well established. In the present study, we investigate the antioxidative capacity of six sunscreen benzophenone compounds. A primary myoblast culture was mixed in vitro with 100 mu M menadione. The cytotoxic effect by menadione-induced oxidative stress was monitored by the lucigenin- or luminol-amplified chemiluminescence, methylthiotetrazole (MTT) assay, and the antioxidative effects of various benzophenone compounds were evaluated. The results showed that the addition of menadione can induce oxidative stress on myoblasts by superoxide and hydrogen peroxide production, which can be eradicated by superoxide dismutase (SOD) and catalase, respectively, in a dose-dependent mode. The catalase has a protective effect on the cytotoxicity induced by menadione as measured by the MTT assay, while the SOD does not. The selected benzophenones also have a significant scavenging effect on the menadione-induced cell death on the myoblasts. The ortho-dihydroxyl structure and other hydroxy groups in the same ring have a stronger scavenging effect on the superoxide anion on myoblasts; thus, a stable penoxy radical may be formed. The mechanism of this effect remains to be clarified. (C) 1999 Elsevier Science Inc.
Relation:	MOLECULAR HUMAN REPRODUCTION 5(6):513-519
Appears in Collections:	[School of Medicine] Journal articles

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