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資料載入中.....
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請使用永久網址來引用或連結此文件:
http://ir.cmu.edu.tw/ir/handle/310903500/30588
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| 題名: | Inhibitory effects of (+/-)-tetrahydropalmatine on thyrotropin-stimulating hormone concentration in hyperthyroid rats |
| 作者: | Hsieh, MT;Wu, LY |
| 貢獻者: | 藥學院中藥所;Hsieh, MT, CHINA MED COLL,INST CHINESE PHARMACEUT SCI,91 HSIEH SHIH RD,TAICHUNG,TAIWAN |
| 日期: | 1996 |
| 上傳時間: | 2010-09-24 14:58:17 (UTC+8) |
| 出版者: | ROYAL PHARMACEUTICAL SOC GREAT BRITAIN |
| 摘要: | During purification of fungal deoxyribonuclease (DNase) from Syncephalastrum racemosum, a protein which was functionally unknown and persistently existed in the DNase-containing fractions through chromatography over DEAE cellulose, hydroxylapatite, and phenyl-Sepharose was identified. The protein was finally separated from DNase after affinity chromatography on a cibacron blue-Sepharose column and purified to apparent homogeneity after gel chromatography on a Superdex 200 HR column. Ten tryptic peptides of this protein were isolated and sequenced. Searching in the sequence data bank with the aid of the computer pro gram PC/Gene, we found that this protein was highly homologous to aspartic proteinases, such as pepsin and rhizopuspepsin. Because of its fungal origin and because the protein indeed showed catalytic cleavage on peptide bonds of bovine serum albumin, RNase, and carbonic anhydrase, we termed this protein syncephapepsin. The molecular weight of syncephapepsin is 38,000 daltons, based on gel filtration and sodium dodecyl sulfate-polyacrylamide electrophoresis. (C) 1996 Academic Press, Inc. |
| 關聯: | JOURNAL OF PHARMACY AND PHARMACOLOGY 48(9):959-961 |
| 顯示於類別: | [中國藥學研究所(已停用)] 期刊論文
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