The stability of cytokeratin 18 in human liver cells during colchicine-induced microtubule disruption

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Title:	The stability of cytokeratin 18 in human liver cells during colchicine-induced microtubule disruption
Authors:	Liu, YH;Su, B;Pei, RJ;Yeh, CC;Yeh, KT;Lee, KY;Hsu, YH;Ho, CC;Lai, YS
Contributors:	醫學院醫學系病理學科;China Med Coll, Sch Med, Dept Pathol, Taichung, Taiwan;China Med Coll Hosp, Dept Urol, Taichung, Taiwan;Changha Christian Hosp, Dept Pathol, Changhua, Taiwan;Jen Ai Hosp, Dept Pathol, Taichung, Taiwan;Tzu Chi Hosp, Dept Pathol, Hua Lien, Taiwan;China Med Coll, Sch Med, Dept Physiol, Taichung, Taiwan
Date:	2001
Issue Date:	2010-09-24 14:57:52 (UTC+8)
Publisher:	PERGAMON-ELSEVIER SCIENCE LTD
Abstract:	By using mRNA differential display to examine specimens of non-small cell lung cancer (NSCLC), we have identified overexpression of dihydrodiol dehydrogenase (DDH) that was not detected in the corresponding normal lung tissue. Normally DDH is associated with catalysis of polycyclic die aromatic hydrocarbons (PAHs) in the liver; in NSCLC cells, DDH expression would implicate an association with disease progression. In this study we investigated the prognostic significance of DDH expression in patients with NSCLC, By using immunohistochemistry, we measured DDH expression in 381 patients with NSCLC, The relationship between DDH expression and clinicopathological parameters (age, gender, smoking history, mitotic index, histological type, stage, cell differentiation, and lymphovascular invasion) was analyzed by chi (2) analysis. Survival curves mere plotted with the method of Kaplan-Meier, and statistical difference of survivals between different groups was compared by a log-rank test. Our results showed that DDH overexpression could be detected in 317 (83.2%) of 381 pathological sections and in 77.9% (60 of 77) of metastatic lymph nodes. Expression of DDH was confirmed by immunoblotting. Compared with patients with DDH overexpression in tumors, patients with low DDH expression had significantly lower incidence of early tumor recurrence and distant organ metastasis (46.7 versus 29.7%; P = 0.045), Interestingly, survival was also significantly better in patients with low DDH expression than in those with DDH overexpression (P = 0.0017), Using univariate analysis, we correlated three important factors, DDH overexpression, tumor stages, and gender, with poor prognosis for NSCLC patients. Nevertheless, biological function and involvement of DDH in the disease progression of NSCLC require additional studies.
Relation:	FOOD AND CHEMICAL TOXICOLOGY 39(1):85-89
Appears in Collections:	[School of Medicine] Journal articles

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