中國醫藥大學機構典藏 China Medical University Repository, Taiwan:Item 310903500/30496
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    题名: The influences of blood glucose and duration of fasting on myocardial glucose uptake of [F-18]fluoro-2-deoxy-D-glucose
    作者: Ding, HJ;Shiau, YC;Wang, JJ;Ho, ST;Kao, A
    贡献者: 附設醫院醫研部;Kao, A, China Med Coll Hosp, Dept Med Res, 2 Yuh Der Rd, Taichung 404, Taiwan
    日期: 2002
    上传时间: 2010-09-24 14:56:28 (UTC+8)
    出版者: LIPPINCOTT WILLIAMS & WILKINS
    摘要: Aims: To isolate a Xanthomonas campestris strain that can use lactose directly for xanthan gum production. Methods and Results: The presence of indigenous beta-galactosidase gene in the wild-type Xc17 was detected by PCR and Southern hybridization. Treatment of Xc17 with nitrous acid resulted in the isolation of Xc17L with a 3.5-fold elevation of beta-galactosidase activity capable of growing in lactose-based medium. Xc17L is stable for at least 100 generations in terms of beta-galactosidase expression. The amounts of xanthan produced by Xc17L in lactose-based medium are comparable to those in glucose-based medium. Conclusions: Xc17L is potentially useful for xanthan production from whey, a waste containing lactose. Significance and Impact of the Study: A lactose-utilizing strain of X. campestris strain can be constructed without incorporation of any exotic DNA or antibiotic resistance gene and therefore concern of a gene-modified organism and fear of a spread of an antibiotic-resistant gene are avoided.
    關聯: NUCLEAR MEDICINE COMMUNICATIONS 23(10):961-965
    显示于类别:[台中附設醫院] 期刊論文

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