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請使用永久網址來引用或連結此文件:
http://ir.cmu.edu.tw/ir/handle/310903500/30475
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| 題名: | Proteolysis of integrin alpha 5 and beta 1 subunits involved in retinoic acid-induced apoptosis in human hepatoma Hep3B cells |
| 作者: | Hsu, SL;Cheng, CC;Shi, YR;Chiang, CW |
| 貢獻者: | 附設醫院醫研部;Taichung Vet Gen Hosp, Dept Educ & Res, Taichung 40705, Taiwan;China Med Coll Hosp, Dept Med Res, Taichung, Taiwan;Vanderbilt Univ, Dept Pediat, Nashville, TN USA;Vanderbilt Univ, Vanderbilt Ingram Canc Ctr, Nashville, TN USA |
| 日期: | 2001 |
| 上傳時間: | 2010-09-24 14:56:05 (UTC+8) |
| 出版者: | ELSEVIER SCI IRELAND LTD |
| 摘要: | The effects of butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) on N-acetyltransferase (NAT) activity were examined using a human colon tumor cell line (colo 205). BHA or BHT were added to the cytosols or to the medium of human colon rumor cells: The NAT activity was measured by high performance liquid chromatography, assaying the amounts of acetylated 2-aminofluorene (AAF), p aminobenzoic acid (N-Ac-PABA), nonacetylated 2 amino-fluorene (AF) and p-aminobenzoic acid (PABA). The NAT activity in the human colon tumor cells and cytosols was suppressed by BHA or BHT in a close-dependent manner. The apparent values of Km and Vmax of NAT of human colon tumor cells were also decreased by BHA or BHT in cytosols and in intact cells. BHA ol BHT may act as a noncompetitive mhibitor. After the incubation of human colon tumor cells with AF in the presence of BHA or BHT, the cells were recovered and DNA was prepared and hydrolysed to nucleotides. Adducted nucleotides were extracted into butanol and AF-DNA adducts were analysed by HPLC. The results also demonstrated that when BHA or BHT was added to the media, a decrease in AF-DNA adduct formation was seen in the human colon tumor cells. The finding of AF-DNA adduct formation in cultured human colon tumor cells suggest the possibility of using cultured cells for assessing arylamine-induced DNA damage. |
| 關聯: | CANCER LETTERS 167(2):193-204 |
| 顯示於類別: | [台中附設醫院] 期刊論文
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