Mutation analysis of the PTEN/MMAC1 gene in cancers of the digestive tract

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Title:	Mutation analysis of the PTEN/MMAC1 gene in cancers of the digestive tract
Authors:	Chang, JG;Chen, YJ;Perng, LI;Wang, NM;Kao, MC;Yang, TY;Chang, CP;Tsai, CH
Contributors:	附設醫院醫研部;China Med Coll Hosp, Dept Med Res, Div Mol Med, Taichung, Taiwan;Mackay Mem Hosp, Dept Med Res, Div Mol Med, Taipei, Taiwan;Taipei Municipal Jen Ai Hosp, Dept Mol Med, Taipei, Taiwan;Taipei Municipal Jen Ai Hosp, Dept Surg, Taipei, Taiwan
Date:	1999
Issue Date:	2010-09-24 14:54:33 (UTC+8)
Publisher:	PERGAMON-ELSEVIER SCIENCE LTD
Abstract:	Diallyl sulfide (DAS) and diallyl disulfide (DADS) were used to determine viability and inhibition of arylamine N-acetyltransferase (NAT) activity in human bladder tumor cells. The NAT activity was measured by high performance liquid chromatography assaying for the amounts of N-acetyl-2-aminofluorene (2-AAF) and N-acetyl-p-aminobenzoic acid (N-Ac-PABA) and remaining 2-aminofluorene (2-AF) and p-aminobenzoic acid (PABA). The viability, NAT activity and 2-AAF-DNA adduct formation in human bladder tumor cells was inhibited by DAS and DADS in a dose-dependent manner, i.e, the higher the concentration of DAS and DADS, the higher the inhibition of NAT activity and cell death. The data also indicated that DAS and DADS decrease the apparent values of Km and Vmax from human, bladder tumor cells in both systems examined. This report is the first demonstration to show garlic components did affect human bladder tumor cell NAT activity.
Relation:	EUROPEAN JOURNAL OF CANCER 35(4):647-651
Appears in Collections:	[China Medical University Hospital] Jurnal articles

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