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請使用永久網址來引用或連結此文件:
http://ir.cmu.edu.tw/ir/handle/310903500/30374
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| 題名: | P53 codon 72Arg polymorphism is not a risk factor for carcinogenesis in the Chinese |
| 作者: | Wang, NM;Tsai, CH;Yeh, KT;Chen, SJ;Chang, JG |
| 貢獻者: | 附設醫院醫研部;China Med Coll Hosp, Div Mol Med, Dept Lab Med, Taichung, Taiwan;China Med Coll Hosp, Div Mol Med, Dept Med Res, Taichung, Taiwan;Changhua Christian Hosp, Dept Pathol, Changhua, Taiwan |
| 日期: | 1999 |
| 上傳時間: | 2010-09-24 14:54:16 (UTC+8) |
| 出版者: | PROFESSOR D A SPANDIDOS |
| 摘要: | Objectives: To demonstrate the effectiveness of ultrarapid cryopreservation inhuman embryos. Design: Retrospective study. Setting: An IVF unit of a medical center. Patient(s): All cases received ultrarapid freezing (URF) of embryos and frozen embryo transfer. Intervention(s): Embryos were placed in phosphate-buffered saline (PB1) + 20% maternal serum (MS) for 5 minutes. Embryos were loaded to the straws containing PB1 + MS + 0.25 M sucrose (SUC) + 3.5 M dimethyl sulfoxide for 3 minutes, and then were stored in a liquid nitrogen tank. Embryos were thawed in a 37 degrees C water for 6 seconds and then cultured in PB1 + MS + SUC for 10 minutes. Embryos were transferred into PB1 + MS for 5 minutes and were transferred into patients. Main Outcome Measure(s): The embryo grades before and after URF, the survival and transferred embryo numbers, and the pregnancy and abortion rates were analyzed. Result(s): A total of 1,582 embryos were thawed, of which 1,273 (80.5%) embryos were transferred and 1,032 (65.2%) embryos survived with greater than or equal to 50% intact blastomere. The embryo numbers with grade I or II before and after freezing and thawing were 1,110 (70.2%) and 790 (50.0%). The mean embryo numbers per transfer was 5.0. Twenty-eight pregnancies (11.4% per transfer) were established, which included 5 miscarriages, 1 ectopic pregnancy, 4 preterm and 18 term deliveries. Conclusion(s): Ultrarapid freezing is worthy of reconsideration for embryo cryopreservation. (C) 1999 by American Society for Reproductive Medicine. |
| 關聯: | INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE 4(3):249-252 |
| 顯示於類別: | [台中附設醫院] 期刊論文
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