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http://ir.cmu.edu.tw/ir/handle/310903500/30365
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| 題名: | Molecular analysis of secretor type alpha(1,2)-fucosyltransferase gene mutations in the Chinese and Thai populations |
| 作者: | Chang, JG;Yang, TY;Liu, TC;Lin, TP;Hu, CJ;Kao, MC;Wang, NM;Tsai, FJ;Peng, CT;Tsai, CH |
| 貢獻者: | 附設醫院醫研部;China Med Coll Hosp, Dept Med Res & Pediat, Div Mol Med, Taichung, Taiwan;Kaohsiung Med Coll Hosp, Dept Internal Med, Div Hematol Oncol, Kaohsiung, Taiwan;Taipei Municipal Jen Ai Hosp, Dept Clin Pathol, Blood Bank Serv, Taipei, Taiwan |
| 日期: | 1999 |
| 上傳時間: | 2010-09-24 14:54:05 (UTC+8) |
| 出版者: | AMER ASSOC BLOOD BANKS |
| 摘要: | Background: When confluent periodontal ligament (PDL) cells were cultured in the presence of dexamethasone (Dex), ascorbic acid (AA), and beta-glycerophosphate (GP), they underwent sequential differentiation, demonstrating distinct morphological characteristics. At 1 week, localized cell proliferation led to the formation of multilayers of cells. As cell differentiation progressed, they formed nodules by deposition of matrix in the clusters of cells at 2 weeks, and mineralized the nodules at 3 weeks. These changes implicate extensive cell-to-cell interactions, Cadherins are known to play an important role in establishing cell contacts during tissue formation. Methods: To determine whether cadherins are involved in PDL cell differentiation, and the formation and mineralization of nodules by the cells in vitro, we investigated the expression of N-cadherin using immunofluorescence labeling and Northern blot analysis. Results: Immunolabeling showed that N-cadherin was expressed in PDL cells in the stages of nodule formation and mineralization. Northern blot analysis demonstrated a 3-fold increase in the expression of N-cadherin mRNA in the stages. However, neither E-cadherin nor P-cadherin was expressed. Conclusions: Our data suggest that N-cadherin may play an important role in PDL cell differentiation and the formation of mineralized nodules by PDL cells. |
| 關聯: | TRANSFUSION 39(9):1013-1017 |
| 顯示於類別: | [台中附設醫院] 期刊論文
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