Sequence polymorphism of mitochondrial D-loop DNA in the Taiwanese Han population

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Title:	Sequence polymorphism of mitochondrial D-loop DNA in the Taiwanese Han population
Authors:	Tsai, LC;Lin, CY;Lee, JCI;Chang, JG;Linacre, A;Goodwin, W
Contributors:	附設醫院檢驗醫學部;Cent Police Univ, Dept Forens Sci, Tao Yuan 33334, Taiwan;China Med Coll Hosp, Dept Lab Med, Taichun, Taiwan;Univ Strathclyde, Dept Pure & Appl Chem, Forens Sci Unit, Glasgow G1 1XW, Lanark, Scotland;Univ Glasgow, Human Identificat Ctr, Dept Forens Med & Sci, Glasgow G12 8QQ, Lanark, Scotland
Date:	2001
Issue Date:	2010-09-24 14:53:25 (UTC+8)
Publisher:	ELSEVIER SCI IRELAND LTD
Abstract:	AIM: An efficient, precise, and sensitive method for identifying Atractylodes plants has been established and will contribute significantly to quality control and scientific analysis in Chinese traditional medicine. METHODS: Twenty primers were applied for setting up the RAPD (randomly amplified polymorphic DNA) markers of Atractylodes plants, Atractylodes lancea DC (A lancea MG:), Atractylodes japonica Koidz (A japonica K), and Atractylodes ovata DC (A ovata DC). The primer OPF03, OPF05, and OPF14 could discriminate them successfully. The results were also able to apply on the Chinese formulations with Atractylodes purchased from local markets. RESULTS: RAPD was used to investigate phylogenetic relationships among and within closely related species. RAPD analysis reflects heritable changes in the nucleotides sequence in both the coding and noncoding regions, because it is conducted directly from the DNA level. This work first conducted RAPD analysis of Atractylodes plants to establish their RAPD makers. CONCLUSION: The RAPD markers could be applied extensively in the Chinese herbal formulations.
Relation:	FORENSIC SCIENCE INTERNATIONAL 119(2):239-247
Appears in Collections:	[China Medical University Hospital] Jurnal articles

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