Effects of noise on monoamine levels in the rat brain using in vivo microdialysis

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Title:	Effects of noise on monoamine levels in the rat brain using in vivo microdialysis
Authors:	Tsai, HY;Lu, YH;Wu, CR;Chen, YF
Contributors:	醫學院醫研所;China Med Univ, Inst Med Sci, Taichung, Taiwan;China Med Univ, Dept Pharmacol, Taichung, Taiwan;China Med Univ, Inst Chinese Pharmaceut Sci, Taichung, Taiwan;China Med Univ Hosp, Dept Pharm, Taichung, Taiwan
Date:	2005
Issue Date:	2010-09-24 14:52:58 (UTC+8)
Publisher:	SPRINGER
Abstract:	Acrylamide has been reported to be present in high-temperature processed foods and normal processed food intake could lead to significant acrylamide exposure. Acrylamide in vivo can be conjugated with glutathione in the presence of glutathione transferase. This conjugation product is further metabolized and excreted as N-acetyl-S-(propionamide)cysteine (NASPC) in the urine. NASPC could be considered a biomarker for acrylamide exposure. The objective of this study was to develop a highly specific, rapid and sensitive method to quantify urinary NASPC, serving as a biomarker for acrylamide exposure assessment. Isotope-labeled [C-13(3)]NASPC was successfully synthesized and used as an internal standard. This urine mixture was directly analyzed using a newly developed liquid chromatographic/tandem mass spectrometric method coupled with an on-line clean-up system. The detection limit for this method was estimated as &LT; 5 μ g l(-1)(0.4 pmol) on-column. The method was applied to measure the urinary level of NASPC in 70 apparently health subjects. The results showed that the NASPC urinary level was highly associated with smoking. Smokers had a significantly higher urinary NASPC level (135 &PLUSMN; 88 μ g g(-1) creatinine) than non-smokers (76 &PLUSMN; 30 μ g g(-1) creatinine). A highly sensitive and selective LC/MS/MS isotope dilution method was successfully established. With an on-line clean-up system, this system is capable of routine high-throughput analysis and accurate quantitation of NASPC in urine. This could be a useful tool for health surveillance for acrylamide exposure in a population for future study. Copyright &COPY; 2005 John Wiley & Sons, Ltd.
Relation:	PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY 450(2):83-87
Appears in Collections:	[Graduate Institute of Medical Science] Journal articles

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