Glycine transporter I inhibitor, N-methylglycine (Sarcosine), added to antipsychotics for the treatment of schizophrenia

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Title:	Glycine transporter I inhibitor, N-methylglycine (Sarcosine), added to antipsychotics for the treatment of schizophrenia
Authors:	Tsai, GC;Lane, HY;Yang, PC;Chong, MY;Lange, N
Contributors:	附設醫院精神醫學部;McLean Hosp, Lab Mol & Psychiat Neurosci, Belmont, MA 02478 USA;McLean Hosp, Lab Stat Neuroimaging, Belmont, MA 02478 USA;Harvard Univ, Sch Med, Boston, MA 02115 USA;China Med Coll Hosp, Dept Psychiat, Taichung, Taiwan;Kaohsiung Med Univ, Dept Psychiat, Kaohsiung, Taiwan
Date:	2004
Issue Date:	2010-09-24 14:52:34 (UTC+8)
Publisher:	ELSEVIER SCIENCE INC
Abstract:	Human epidemiological studies suggest an association between N-acelyltransferase (NAT) activity and the incidence of bladder and colorectal cancers. In this study, paclitaxel was selected to examine the inhibition of arylamine NAT activity, gene expression and 2-aminofluorene-DNA adduct formation in a human osteogenic sarcoma cell line (U-2 OS). The activity of NAT was determined by high performance liquid chromatography (HPLC) assay for the amounts of acetylated 2-aminofluorene (AF) and p-aminobenzoic acid (PABA) and nonaceylated AF and PABA. Human osteogenic sarcoma cell cytosols and intact cells were used to examine the NAT activity, gene expression and AF-DNA adduct formation. The results demonstrated that NAT activity, percent of NAT in examined cells, gene expression (NAT] mRNA) and AF-DNA adduct formation in human osteogenic sarcoma cells were inhibited and decreased by paclitaxel in a dose-dependent manner. The results also demonstrated that paclitaxel decreased the apparent values of Km and Vmax from intact human osteogenic sarcoma cells (U-2 OS). Thus, paclitaxel is an uncompetitive inhibitor of the NAT enzyme.
Relation:	BIOLOGICAL PSYCHIATRY 55(5):452-456
Appears in Collections:	[China Medical University Hospital] Jurnal articles

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