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| 題名: | Dose-dependent alternations in the pharmacokinetics of olanzapine during coadministration of fluvoxamine in patients with schizophrenia |
| 作者: | Chiu, CC;Lane, HY;Huang, MC;Liu, HC;Jann, MW;Hon, YY;Chang, WH;Lu, ML |
| 貢獻者: | 附設醫院精神醫學部;Taipei Med Univ, Wan Fang Hosp, Dept Psychiat, Taipei, Taiwan;Taipei City Psychiat Ctr, Lab Biol Psychiat, Taipei, Taiwan;China Med Univ & Hosp, Dept Psychiat, Taichung, Taiwan;Hung Chi Psychiat Hosp, Taipei, Taiwan;Mercer Univ, So Sch Pharm, Dept Clin & Adm Sci, Atlanta, GA USA;Natl Inst Hlth, Ctr Clin, Dept Pharm, Clin Pharmacokinet Res Lab, Bethesda, MD USA;Tzu Chi Univ, Hualien, Taiwan;Dalin Tzu Chi Gen Hosp, Dept Psychiat, Hualien, Taiwan |
| 日期: | 2004 |
| 上傳時間: | 2010-09-24 14:52:24 (UTC+8) |
| 出版者: | SAGE PUBLICATIONS INC |
| 摘要: | In this study, diallyl sulfide (DAS), diallyl disulfide (DADS) and diallyl trisulfide (DATS), which are major organosulfur compounds (OSCs) of garlic, were used as experimental materials to investigate their modulation effects on cell viability and cell cycle in human liver tumor cells (J5). According to the results of cell viability assay, 50 or 100 muM DATS significantly decreased the cell viability as compared with the control (P < 0.05) in dose and time dependent relations. Phenomena of cell number loss, shape deformation and lysis were observed after treatment with 100 muM DATS for 24 h. Cell cycle studies showed that J5 cells were significantly arrested in G2/M phase as the cells were treated with 100 muM DADS, 10, 50 or 100 muM DATS for 24 h (P < 0.05). DATS was more effective in arresting cells in G2/M phase than DADS, and the phenomena of arresting J5 cells in G2/M phase increased obviously in dose and time dependent relations. According to the Western blot analysis, DATS decreased cyclin-dependent kinase (Cdks)-Cdk7 (i.e. Cdc2 activate kinase) protein levels in J5 cells but increased cyclin B1 protein level. The modulation potency to cyclin B1 and Cdk7 expressions was in the order of DATS>DADS>DAS. The modulation potency to cyclin B1 and Cdk7 protein levels increased with increasing in DATS concentration and culture time. In conclusion, DATS might affect cell viability and cell morphological changes in J5 cells and lead,cells to be arrested in G2/M phase via controlling the expression of cyclin B1 and Cdk7 in J5 cells; and the controlling action might relate to the sulfuric atom numbers in the structures of all these allyl sulfides. (C) 2004 Elsevier Ltd. All rights reserved. |
| 關聯: | JOURNAL OF CLINICAL PHARMACOLOGY 44(12):1385-1390 |
| 顯示於類別: | [台中附設醫院] 期刊論文
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