Laser in situ keratomileusis for different degrees of myopia

中國醫藥大學機構典藏 China Medical University Repository, Taiwan > 台中附設醫院 > 期刊論文 > Item 310903500/30212

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题名:	Laser in situ keratomileusis for different degrees of myopia
作者:	Lin, JM;Tsai, YY
贡献者:	附設醫院眼科部;China Med Univ Hosp, Dept Ophthalmol, Taichung 404, Taiwan
日期:	2005
上传时间:	2010-09-24 14:51:13 (UTC+8)
出版者:	BLACKWELL MUNKSGAARD
摘要:	Purpose: To investigate the spectrum of p53 gene mutations and the relationship between gene mutation and p53 protein levels in pterygium. Methods: Pterygial samples were harvested from 51 patients undergoing pterygium surgery. DNA samples for p53 mutation analyses were extracted from epithelial cells and subjected to DNA sequencing for examination of mutations in exons 4, 5, 6, 7, and 8 of the p53 gene. In situ levels of p53 protein were studied by immunohistochemistry (IHC) and the percentage of positively stained cells quantified. Ten normal conjunctiva samples were included in this study as controls. Results: Mutations within the p53 gene were detected in 8 pterygial samples (15.7%) with only one mutation found in each sample. All the mutations observed were point mutations, with 6 being substitutions and 2 deletions. Three mutations were identified in exon 6, two in exon 7, and a single mutation found in each of exons 4, 5, and 8. P53 protein levels were scored as 0 (negative) in 31 pterygial specimens (60.8%), +1 in 9 samples (17.6%), +2 in 5 samples (9.8%), and +3 in 6 samples (11.8%) by IHC. The 8 samples found to have p53 gene mutations were equally distributed among the different levels of p53 protein observed using IHC, with 2 samples in each group. The two deletion mutations, which caused a frame shift to occur, were found in samples negative for p53 immunostaining (score 0), while substitution mutations were found in samples positively stained (score +1, +2, and +3). Conclusions: Mutations within p53 gene exons 4-8 were detected in pterygial epithelium and the mutations showed no correlation with p53 protein levels as seen by IHC.
關聯:	ACTA OPHTHALMOLOGICA SCANDINAVICA 83(1):40-45
显示于类别:	[台中附設醫院] 期刊論文

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