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| 題名: | p53 expression in pterygium by immunohistochemical analysis - A series report of 127 cases and review of the literature |
| 作者: | Tsai, YY;Chang, KC;Lin, CL;Lee, H;Tsai, FD;Cheng, YW;Tseng, SH |
| 貢獻者: | 附設醫院眼科部;Natl Cheng Kung Univ Hosp, Dept Ophthalmol, Tainan, Taiwan;China Med Univ Hosp, Dept Ophthalmol, Taichung, Taiwan;Chung Shan Med Univ, Inst Med, Taichung, Taiwan;Natl Cheng Kung Univ Hosp, Dept Pathol, Tainan, Taiwan;China Med Univ Hosp, Dept Phys Med & Rehabil, Taichung, Taiwan;Chung Shan Med Univ, Inst Toxicol, Taichung, Taiwan;China Med Univ, Dept Med Genet, Taichung, Taiwan;China Med Univ, Coll Chinese Med, Taichung, Taiwan |
| 日期: | 2005 |
| 上傳時間: | 2010-09-24 14:51:05 (UTC+8) |
| 出版者: | LIPPINCOTT WILLIAMS & WILKINS |
| 摘要: | 1 Artocarpol A (ART), a natural phenolic compound isolated from Artocarpus rigida, stimulated a slow onset and long-lasting superoxide anion generation in rat neutrophils, whereas only slightly activated the NADPH oxidase in a cell-free system. 2 Pretreatment of neutrophils with pertussis toxin (1 mu g ml(-1)), 50 mu M 2'-amino-3'-methoxyflavone (PD 98059), or 1 mu M 1,4-diamino-2,3-dicyano-1,4-bis(2-aminophenylthio) butadiene (U0126) had no effect on ART-stimulated superoxide anion generation. ART (30 mu M) did not induce extracellular signal-regulated kinase (ERK) phosphorylation. 3 4-(4-Fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-1H-imidazole (SB 203580) markedly attenuated the ART-stimulated superoxide anion generation (IC50 value of 4.3 +/- 0.3 mu M). Moreover, ART induced p38 mitogen-activated PK ( MAPK) phosphorylation and activation. 4 The superoxide anion generation in response to ART was also substantially inhibited in a Ca2+-free medium, and by pretreatment with 1 mu M 1-[6-((17 beta-3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl]-1H-pyrrole-2,5-dione (U-73122) and 100 mu M 2-aminoethyldiphenyl borate (2-APB). ART (30 mu M) stimulated the [Ca2+](i) elevation in the presence or absence of external Ca2+, and also increased the D-myo-inositol 1,4,5-trisphosphate formation. 5 2-[1-(3-Dimethylaminopropyl)-1H-indol-3-yl]-3-(1H-indol-3-yl)-maleimide (GF 109203X) greatly inhibited the ART-stimulated superoxide anion generation (IC50 value of 7.8 +/- 1.0 nM). ART increased the recruitment of PKC-alpha, -beta I, and -beta II to the plasma membrane of neutrophils, and stimulated Ca2+-dependent PKC activation in the cytosol preparation. 6 ART induced the phosphorylation of p47(phox), which was attenuated by GF 109203X. Moreover, ART evoked the membrane association of p47(phox), which was inhibited by GF 109203X and SB 203580. 7 These results indicate that the ART stimulation of superoxide anion generation involved the activation of p38 MAPK, PLC/Ca2+, and PKC signaling pathways in rat neutrophils. |
| 關聯: | CORNEA 24(5):583-586 |
| 顯示於類別: | [台中附設醫院] 期刊論文
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