A codon 31(ser-arg) polymorphism of the WAF-1/CIP-1/p21/tumour suppressor gene in Chinese primary open-angle glaucoma

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Title:	A codon 31(ser-arg) polymorphism of the WAF-1/CIP-1/p21/tumour suppressor gene in Chinese primary open-angle glaucoma
Authors:	Tsai, FJ;Lin, HJ;Chen, WC;Tsai, CH;Tsai, SW
Contributors:	附設醫院眼科部;China Med Univ Hosp, Dept Ophthalmol, Taichung 404, Taiwan;China Med Univ Hosp, Dept Med Genet & Paediat, Taichung 404, Taiwan;China Med Univ, Inst Environm Med, Dept Occupat Safety & Hlth, Taichung, Taiwan
Date:	2004
Issue Date:	2010-09-24 14:50:37 (UTC+8)
Publisher:	BLACKWELL MUNKSGAARD
Abstract:	Aberrant promoter methylation of CpG islands of tumor suppressor genes inhibits expression of the genes and may lead to tumorigenesis. We investigated the aberrant methylation profile of potential tumor suppressor genes of p15, p16, SOCS-1. and Wnt signaling pathway in colorectal cancers and correlated the data with clinical findings. Cancerous and nearby non-cancerous tissues of 185 sporadic colorectal cancer samples were studied. Methylation specific PCR was performed to explore the mechanism of inactivation in p15, p16 SOCS-1, E-cadherin, APC, GSK-3beta, and Axin 1 genes. Aberrant promoter methylation in p15, p16, SOCS-1, E-cadherin. APC, GSK-3beta, and Axin1 genes were 5.9, 7.0, 3.8, 5.9, 12.4. 2.2, and 0% for cancerous tissues, respectively, whereas the frequencies were 3.8, 0, 0, 7.0, 2.7, 0.5, and 0% for nearby non-cancerous tissues, respectively. The frequency of aberrant promoter methylation of cancerous tissues was significant higher than non-cancerous tissues in p16, SOCS-1, and APC genes (p<0.05) and methylation status of these genes had no clear relationship with clinical parameters. Of the 66 patients who showed at least one aberrant promoter methylation in the tumor-suppressor genes, 5 (7.6%) patients demonstrated multiple methylation phenotype (methylation greater than or equal to3) and associated with increased lymph node metastasis (p=0.036). Our findings suggest that inactivation of some tumor suppressor genes through aberrant promoter methylation of CpG islands may play a role in the development of colorectal cancer and methylation inactivation of these genes except p 16 and SOCS I may occur at the precancerous stage. Multiple methylation pathways may be involved in the tumorigenesis of colorectal cancer and associated with aggressiveness of clinical disease.
Relation:	ACTA OPHTHALMOLOGICA SCANDINAVICA 82(1):76-80
Appears in Collections:	[China Medical University Hospital] Jurnal articles

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