Insulin-like growth factor-II gene polymorphism is associated with primary open angle glaucoma

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Title:	Insulin-like growth factor-II gene polymorphism is associated with primary open angle glaucoma
Authors:	Tsai, FJ;Lin, HJ;Chen, WC;Chen, HY;Fan, SS
Contributors:	附設醫院眼科部;China Med Univ Hosp, Dept Ophthalmol, Taichung 404, Taiwan;China Med Univ Hosp, Dept Med Genet & Pediat, Taichung 404, Taiwan;Tunghai Univ, Inst Biol, Taichung 40704, Taiwan;Tunghai Univ, Life Sci Res Ctr, Taichung 40704, Taiwan
Date:	2003
Issue Date:	2010-09-24 14:50:15 (UTC+8)
Publisher:	WILEY-LISS
Abstract:	The purpose of this study was to prepare and evaluate in vitro the feasibility and cytocompatibility of a novel composite (GGT) as a large defect bone substitute. The composite is tricalcium phosphate ceramic particles combined with genipin crosslinked gelatin. After soaking the GGT composites in Ringer solutions at 37 C for 7, 14, 28, 42, 56, and 84 days, the in vitro biologic degradation rate and biocompatibility were determined. Substances released from soaked GGT composites were analyzed with an ultraviolet visible light spectrophotometer. In addition, the solution soaking the GGT was co-cultured with osteoblasts to determine whether or not the released substances from GGT could facilitate the growth of bone cells. After they had been cultured for 2 days, the osteoblasts were tested for differentiation and proliferation by alkaline phosphatase (ALP) activity and a MTT assay. Results indicate that the concentration of the genipin solution is a critical factor in deciding the crosslinking degree of the GGT composite. Complete crosslinking reaction in the GGT composite occurred when 0.5 wt % of genipin had been added. Cytotoxic testing revealed that 80 ppm of the genipin in the culture medium served as the level over which cytotoxicity to osteoblasts could be produced. In addition, we found that gelatin and calcium continuously were released from the GGT composite in the soaking solution, which promoted differentiation and proliferation of the osteoblasts. (C) 2003 Wiley Periodicals, Inc.
Relation:	JOURNAL OF CLINICAL LABORATORY ANALYSIS 17(6):259-263
Appears in Collections:	[China Medical University Hospital] Jurnal articles

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