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| 題名: | Light-induced changes in frog pineal gland N-acetyltransferase activity |
| 作者: | Lee, JH;Hung, CF;Ho, CC;Chang, SH;Lai, YS;Chung, JG |
| 貢獻者: | 醫學院醫學系;CHINA MED COLL,DEPT MED,TAICHUNG 400,TAIWAN;JEN AI HOSP,DEPT SURG,TAICHUNG,TAIWAN;CHINA MED COLL HOSP,DEPT SURG,TAICHUNG,TAIWAN |
| 日期: | 1997 |
| 上傳時間: | 2010-09-24 14:48:10 (UTC+8) |
| 出版者: | PERGAMON-ELSEVIER SCIENCE LTD |
| 摘要: | Ibuprofen, one of the nonsteroidal anti-inflammatory drugs, inhibited arylamine N-acetyltransferase activity of Klebsiella pneumoniae both in vitro and in vivo. The NAT activities of Klebsiella pneumoniae were inhibited by ibuprofen in a dose-dependent manner both in vitro and in vivo. In vitro, the NAT activity was 0.675 +/- 0.028 nmol/min/mg of protein for the acetylation of 2-aminofluorene. In the presence of 8 mM ibuprofen, the NAT activity was 0.506 +/- 0.002 nmol/min/mg of protein for the acetylation of 2-aminofluorene. In vivo, the NAT activity was 0.279 +/- 0.016 nmol/min/10(10) colony forming units (CFU) for the acetylation of 2-aminofluorene. In the presence of 8 mM ibuprofen, the NAT activity was 0.228 +/- 0.008 nmol/min/10(10) CFU for the acetylation of 2-aminofluorene. The inhibition of NAT activity by ibuprofen was shown to persist for at least 4 h. For in vitro examination, the values of apparent K-m and V-max were 1.08 +/- 0.05 mM and 9.17 +/- 0.11 nmol/min/mg of protein, respectively, for 2-aminofluorene. However, when 8 mM of ibuprofen was added to the reaction mixtures, the values of apparent K-m and V-max were 1.19 +/- 0.01 mM and 6.67 +/- 0.11 nmol/min/mg of protein, respectively, for 2-aminofluorene. For in vivo examination, the values of apparent K-m and V-max were 1.24 +/- 0.48 mM and 4.18 +/- 1.06 nmol/min/10 x 10(10) CFU, respectively, for 2-aminofluorene, However, when 8 mM of ibuprofen was added to the culture, the values of apparent K-m and V-max were 0.95 +/- 0.29 mM and 2.77 +/- 0.37 nmol/min/mg protein, respectively, for 2-aminofluorene, respectively. This report is the first finding of ibuprofen inhibition of arylamine N-acetyltransferase activity in a strain of Klebsiella pneumoniae. |
| 關聯: | NEUROCHEMISTRY INTERNATIONAL 31(4):533-540 |
| 顯示於類別: | [醫學系] 期刊論文
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