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請使用永久網址來引用或連結此文件:
http://ir.cmu.edu.tw/ir/handle/310903500/30038
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| 題名: | Longitudinal Doppler sonographic measurements of vascular impedance in the central and peripheral spiral arteries throughout pregnancy |
| 作者: | Hsieh, YY;Chang, CC;Tsai, HD;Lee, CC;Tsai, CH |
| 貢獻者: | 附設醫院婦產部;China Med Coll Hosp, Dept Obstet & Gynecol, Taichung, Taiwan;China Med Coll Hosp, Dept Pediat, Taichung, Taiwan |
| 日期: | 2000 |
| 上傳時間: | 2010-09-24 14:48:08 (UTC+8) |
| 出版者: | JOHN WILEY & SONS INC |
| 摘要: | Objective. PTEN/MMAC1, a candidate tumor suppressor gene located at chromosome 10q23.3, was recently identified and found to be homozygously deleted or mutated in several different types of human tumors. The aim of this study is to determine whether PTEN/ MMAC1 is a target for 10q loss of heterozygosity in cervical cancer. Method. We examined 50 primary cervical carcinoma specimens using a PCR-based assay followed by SSCP and direct sequencing. The genomic DNA was also confirmed by Southern blot analysis, Results. All specimens except one, which has a 7-base deletion, showed a negative result. Among them, 30 randomly selected cases and their paired noncancerous tissue were further screened using nested RT-PCR. Six of 30 cervical cancerous tissues had aberrant transcripts. However, 4 of the matched noncancerous tissues also had aberrant transcripts. Southern blot analysis of the entire genomic DNA did not reveal any evidence of gene alteration. Conclusions. Sequence abnormalities in the PTEN/MMAC1 gene were only detected in 1 of 50 cervical cancers analyzed indicating that aberrant PTEN/MMAC1 function is an uncommon event in the development of cervix cancers. However, similar to studies with the TSG101 gene, screening for aberrant transcripts of PTEN/MMAC1 with nested RT-PCR may detect transcripts, which, although they vary from the normal size, may not be related to oncogenesis as they are also frequently found in normal tissues of the same patient. (C) 2000 Academic Press. |
| 關聯: | JOURNAL OF CLINICAL ULTRASOUND 28(2):78-82 |
| 顯示於類別: | [台中附設醫院] 期刊論文
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