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請使用永久網址來引用或連結此文件:
http://ir.cmu.edu.tw/ir/handle/310903500/30033
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| 題名: | VOC concentration in Taiwan's household drinking water |
| 作者: | Kuo, HW;Chiang, TF;Lo, LI;Lai, JS;Chan, CC;Wang, JD |
| 貢獻者: | 醫學院醫學系;China Med Coll, Inst Environm Hlth, Taichung, Taiwan;Natl Taiwan Univ, Coll Publ Hlth, Inst Occupat Med & Ind Hyg, Taipei 10764, Taiwan |
| 日期: | 1997 |
| 上傳時間: | 2010-09-24 14:48:03 (UTC+8) |
| 出版者: | ELSEVIER SCIENCE BV |
| 摘要: | Arylamine N-acetyltransferase (NAT) activities with p-aminobenzoic acid and 2-aminofluorene were determined in Helicobacter pylori, a gram-negative rod bacteria collected from peptic ulcer patients. The NAT activity was determined using a acetyl CoA recycling assay and HPLC. Cytosols or suspensions of H. py[ori with and without selected concentrations of emodin co-treatment showed different percentages of 2-aminofluorene and p-aminobenzoic acid acetylation. The data indicate that there were decreased NAT activity associated with increased emodin in H. pylori cytosols. As 400 mu M of emodin can obviously inhibit NAT activity both in vitro and in vivo (inhibition rate 90% and 93% for 2-aminofluorene and p-aminobenzoic acid in vitro, and 90% and 92%, respectively, for both substrate in vivo). For in vitro examination, the apparent values of K-m and V-max were 3.12+/-0.38 mM and 15.20+/-3.16 nmol/min/mg protein for 2-aminofluorene, and 0.56+/-0.12 mM and 0.74+/-0.09 nmol/min/mg protein for p-aminobenzoic acid. However, when emodin was added to the reaction mixtures, the values of apparent K-m and V-max were 2.40+/-0.32 mM and 10.62+/-0.04 nmol/min/mg protein for 2-aminofluorene, and 0.23+/-0.02 mM and 0.62+/-0.08 nmol/min/mg protein for p-aminobenzoic acid. For in vivo examination, the apparent K-m and V-max were 0.82+/-0.18 mM and 0.92+/-0.21 nmol/min/10x10(10) colony forming units (CFU) for 2-aminofluorene, and 0.78+/-0.14 mM and 0.52+/-0.06 nmol/min/10x10(10) (CFU) for p-aminobenzoic acid. However, when emodin was added to the reaction mixtures, the values of apparent K-m and V-max were 0.50+/-0.08 mM and 0.62+/-0.22 nmol/min/10x10(10) (CFU) for 2-aminofluorene, and 0.52+/-0.21 mM and 0.26+/-0.04 nmol/min/10x10(10) (CFU) for p-aminobenzoic acid. This report is the first finding of emodin inhibition of arylamine N-acetyltransferase activity in a strain of H. pylori. (C) 1997 Elsevier Science Ltd. All rights reserved. |
| 關聯: | SCIENCE OF THE TOTAL ENVIRONMENT 208(1月2日):41-47 |
| 顯示於類別: | [醫學系] 期刊論文
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