Effect of different concentrations of recombinant leukemia inhibitory factor on different development stage of mouse embryo in vitro

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Title:	Effect of different concentrations of recombinant leukemia inhibitory factor on different development stage of mouse embryo in vitro
Authors:	Tsai, HD;Chang, CC;Hsieh, YY;Hsu, LW;Chang, SC;Lo, HY
Contributors:	附設醫院婦產部;China Med Coll Hosp, Dept Obstet & Gynecol, Taichung, Taiwan
Date:	2000
Issue Date:	2010-09-24 14:47:33 (UTC+8)
Publisher:	KLUWER ACADEMIC/PLENUM PUBL
Abstract:	The gram-negative plant pathogen Xanthomonas campestris pv. vesicatoria strain Xv2 harbors an indigenous, cryptic plasmid pXV2 of 14.6 kb. This plasmid can only be maintained in Xanthomonas and is incapable of self-transmission. However, incompatibility testing classified it in IncW, a group containing the smallest number of naturally occurring, broad-host-range, conjugative plasmids. A pXV2 derivative containing, only a 5.5-kb PstI fragment is stably maintained. Deletion of a 3.0-kb region from the PstI fragment causes a loss of plasmid stability. Nucleotide sequencing of the 3.0-kb region essential for autonomous replication revealed a repA gene and a downstream noncoding region containing foul iterons, two 17- and two 19-nt direct repeats, and an AT-rich region lying between the two sets of iterons. The sequence of the deduced RepA and the iterons shows homology to the RepA (39% identity) and the iterons, respectively, of the IncW plasmid pSa. Maxicell expression of the repA gene produced a protein of 35 kDa, a size similar to that deduced from the nucleotide sequence. Trans-complementation test confirmed that the repA gene and the iterons are indeed the essential elements for pXV2 replication. (C) 2000 Academic Press.
Relation:	JOURNAL OF ASSISTED REPRODUCTION AND GENETICS 17(6):352-355
Appears in Collections:	[China Medical University Hospital] Jurnal articles

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