Doppler evaluation of the uterine and spiral arteries from different sampling sites and phases of the menstrual cycle during controlled ovarian hyperstimulation

中國醫藥大學機構典藏 China Medical University Repository, Taiwan > 台中附設醫院 > 期刊論文 > Item 310903500/29997

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题名:	Doppler evaluation of the uterine and spiral arteries from different sampling sites and phases of the menstrual cycle during controlled ovarian hyperstimulation
作者:	Hsieh, YY;Chang, FCC;Tsai, HD
贡献者:	附設醫院婦產部;China Med Coll Hosp, Dept Obstet & Gynecol, Taichung, Taiwan
日期:	2000
上传时间:	2010-09-24 14:47:23 (UTC+8)
出版者:	BLACKWELL SCIENCE LTD
摘要:	AIM: The effect of trans-cinnamaldehyde (CNMA) on the release of norepinephrine (NE) from nerve terminal was investigated using rat pheochromocytoma 12 (PC-12) cells. METHODS: The amount of NA released from PC-12 cells incubated with CNMA or related substances was quantified by high performance liquid chromatography (HPLC)-electrochemical detection. The, lipophilic anion bisoxonol was used to monitor the effect of CNMA on the membrane potential. RESULTS: CNMA; stimulated the secretion of NE in a concentration-dependent manner from 5 mu mol/L to 50 mu mol/L, while the value of lactate dehydrogenase in the incubated medium was not influenced by CNMA. However, acetaldehyde, cinnamic acid, cinnamoyl chloride and cinnamamide failed to produce similar effect. The action of CNMA can thus be considered specific. The depolarizing effect of CNMA on the membrane potential was also illustrated by a concentration-dependent increase in the fluorescence of bisoxonol, a potential-sensitive dye. Saxitoxin attenuated the depolarizing action of CNMA at concentrations sufficient to block sodium channels. Besides, the effect of CNMA to depolarize the membrane potential in PC-12 cells is greater than that of 4-aminopyridine (4-AP). The action of CNMA on NE releasing depends on extracellular Ca2+ and is attenuated by 8-bromo-cAMP at concentrations sufficient to inhibit the action of cyclic AMP. CONCLUSION: These findings suggest that CNMA can depolarize the membrane to result in a Ca2+-dependent and cyclic AMP-related release bf NE from PC-12 cells.
關聯:	ULTRASOUND IN OBSTETRICS & GYNECOLOGY 16(2):192-196
显示于类别:	[台中附設醫院] 期刊論文

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