Doppler evaluation of the uterine and spiral arteries from different sampling sites and phases of the menstrual cycle during controlled ovarian hyperstimulation

CMUR > China Medical University Hospital > Jurnal articles > Item 310903500/29996

Please use this identifier to cite or link to this item: http://ir.cmu.edu.tw/ir/handle/310903500/29996

Title:	Doppler evaluation of the uterine and spiral arteries from different sampling sites and phases of the menstrual cycle during controlled ovarian hyperstimulation
Authors:	Hsieh, YY;Chang, FCC;Tsai, HD
Contributors:	附設醫院婦產部;China Med Coll Hosp, Dept Obstet & Gynecol, Taichung, Taiwan
Date:	2000
Issue Date:	2010-09-24 14:47:22 (UTC+8)
Publisher:	BLACKWELL SCIENCE LTD
Abstract:	AIM: The effect of trans-cinnamaldehyde (CNMA) on the release of norepinephrine (NE) from nerve terminal was investigated using rat pheochromocytoma 12 (PC-12) cells. METHODS: The amount of NA released from PC-12 cells incubated with CNMA or related substances was quantified by high performance liquid chromatography (HPLC)-electrochemical detection. The, lipophilic anion bisoxonol was used to monitor the effect of CNMA on the membrane potential. RESULTS: CNMA; stimulated the secretion of NE in a concentration-dependent manner from 5 mu mol/L to 50 mu mol/L, while the value of lactate dehydrogenase in the incubated medium was not influenced by CNMA. However, acetaldehyde, cinnamic acid, cinnamoyl chloride and cinnamamide failed to produce similar effect. The action of CNMA can thus be considered specific. The depolarizing effect of CNMA on the membrane potential was also illustrated by a concentration-dependent increase in the fluorescence of bisoxonol, a potential-sensitive dye. Saxitoxin attenuated the depolarizing action of CNMA at concentrations sufficient to block sodium channels. Besides, the effect of CNMA to depolarize the membrane potential in PC-12 cells is greater than that of 4-aminopyridine (4-AP). The action of CNMA on NE releasing depends on extracellular Ca2+ and is attenuated by 8-bromo-cAMP at concentrations sufficient to inhibit the action of cyclic AMP. CONCLUSION: These findings suggest that CNMA can depolarize the membrane to result in a Ca2+-dependent and cyclic AMP-related release bf NE from PC-12 cells.
Relation:	ULTRASOUND IN OBSTETRICS & GYNECOLOGY 16(2):192-196
Appears in Collections:	[China Medical University Hospital] Jurnal articles

Files in This Item:

There are no files associated with this item.

Loading...