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請使用永久網址來引用或連結此文件:
http://ir.cmu.edu.tw/ir/handle/310903500/29992
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| 題名: | Prenatal diagnosis of dyssegmental dysplasia - A case report |
| 作者: | Hsieh, YY;Chang, CC;Tsai, HD;Lee, CC;Tsai, FJ;Tsai, CH |
| 貢獻者: | 附設醫院婦產部;China Med Coll Hosp, Dept Obstet & Gynecol, Taichung, Taiwan;China Med Coll Hosp, Dept Pediat, Taichung, Taiwan |
| 日期: | 1999 |
| 上傳時間: | 2010-09-24 14:47:18 (UTC+8) |
| 出版者: | SCI PRINTERS & PUBL INC |
| 摘要: | Arylamine N-acetyltransferase (NAT) activities with 2-aminofluorene (2-AF) were determined in the bacterium Klebsiella pneumoniae. Cytosols or suspensions of K, pneumoniae with or without specific concentrations of diallyl sulphide (DAS) or diallyl disulphide (DADS) as co-treatment showed different percentages of 2-AF acetylation, The data indicated that there was decreased NAT activity associated with increased levels of DAS or DADS in K, pneumoniae, In growth studies on K, pneumoniae it was demonstrated that DAS or DADS elicited a dose-dependent bacteriocide effect on K, pneumoniae, For the cytosol examinations, the apparent values of K-m and V-max were 0.96 +/- 0.09 mM and 7.87 +/- 0.79 nmol min(-1) mg(-1) protein, respectively, for 2-AF, However, when DAS or DADS was added to the reaction mixtures, the apparent values of K-m and V-max were 0.16 +/- 0.04 mM and 0.99 +/- 0.16 nmol min(-1) mg(-1) protein with DAS, respectively, and 0.14 +/- 0.18 mM and 0.85 +/- 0.10 nmol min(-1) mg(-1) protein with DADS, respectively, for 2-AF, For the intact bacteria examination, the apparent values of K-m and V-max were 0.57 +/- 0.06 mM and 2.00 +/- 0.14 nmol min(-1) per 10 x 10(10) CFU, respectively, for 2-AF, However, when DAS or DADS was added to the reaction mixtures, the apparent of values of K-m and V-max were 0.41 +/- 0.04 mM and 1.30 +/- 0.10 nmol min(-1) per 10 x 10(10) CFU with DAS, respectively, and 0.34 +/- 0.04 mM and 1.08 +/- 0.08 nmol min(-1) per 10 x 10(10) CFU with DADS, respectively, for 2-AF, This report is the first demonstration to show that the garlic components DAS and DADS would affect K, pneumoniae growth and NAT activity. Copyright (C) 1999 John Wiley & Sons, Ltd. |
| 關聯: | JOURNAL OF REPRODUCTIVE MEDICINE 44(3):303-305 |
| 顯示於類別: | [台中附設醫院] 期刊論文
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