中國醫藥大學機構典藏 China Medical University Repository, Taiwan:Item 310903500/29978
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 29490/55136 (53%)
造访人次 : 1555390      在线人数 : 65
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 查询小技巧:
  • 您可在西文检索词汇前后加上"双引号",以获取较精准的检索结果
  • 若欲以作者姓名搜寻,建议至进阶搜寻限定作者字段,可获得较完整数据
  • 进阶搜寻
    主页登入上传说明关于CMUR管理 到手机版


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ir.cmu.edu.tw/ir/handle/310903500/29978


    题名: Bilateral simultaneous tubal sextuplets: pregnancy after in-vitro fertilization-embryo transfer following salpingectomy
    作者: Chang, CC;Wu, TH;Tsai, HD;Lo, HY
    贡献者: 附設醫院婦產部;China Med Coll Hosp, Dept Obstet & Gynecol, Invitro Fertilizat Unit, Taichung, Taiwan
    日期: 1998
    上传时间: 2010-09-24 14:47:03 (UTC+8)
    出版者: OXFORD UNIV PRESS
    摘要: Syncephapepsin is a fungal aspartic proteinase from Syncephalastrum racemosum. By using the property of syncephapepsin after having increased activity at higher temperature, two rapid purification protocols were developed, In the former case, a crude extract was initially diluted fivefold with an activity assay buffer and heated at 50 degrees C overnight, In this situation, syncephapepsin would digest most of the proteins that the crude extract contained, Subsequently, syncephapepsin of the crude extract was precipitated from 50 to 70% of ammonium sulfate and the preparation was then directly applied to the Superdex 200 HR FPLC column. In this manner, syncephapepsin was rapidly purified to apparent homogeneity within 24 h. In this report, an alternative method of purification is also provided, Compared with the procedure mentioned above, the heating step was proceeded after FPLC chromatography through which the same result was obtained. Using cytochrome c and RNase A as substrates, the cleavage sites of both substrates were identified by HPLC peptide mapping. The results showed that syncephapepsin had a broad specificity. Residues recognized to be cleaved were primarily those of trypsin and chymotrypsin and Lys was the most susceptible, (C) 1998 Academic Press.
    關聯: HUMAN REPRODUCTION 13(3):762-765
    显示于类别:[台中附設醫院] 期刊論文

    文件中的档案:

    没有与此文件相关的档案.



    在CMUR中所有的数据项都受到原著作权保护.

    TAIR相关文章

     


    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈