Association of CYP17 gene polymorphism and rheumatoid arthritis in Chinese patients in central Taiwan

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Title:	Association of CYP17 gene polymorphism and rheumatoid arthritis in Chinese patients in central Taiwan
Authors:	Lo, SF;Huang, CM;Lin, HC;Tsai, CH;Tsai, FJ
Contributors:	附設醫院基因醫學部;China Med Univ Hosp, Dept Med Genet, Taichung, Taiwan;China Med Univ Hosp, Dept Pediat, Taichung, Taiwan;China Med Univ Hosp, Dept Phys Med & Rehabil, Taichung, Taiwan;China Med Univ Hosp, Dept Internal Med, Div Rheumatol & Immunol, Taichung, Taiwan;China Med Univ, Sch Phys Therapy, Taichung, Taiwan;Taichung Healthcare & Management Univ, Taichung, Taiwan;China Med Univ, Coll Chinese Med, Taichung, Taiwan
Date:	2005
Issue Date:	2010-09-24 14:46:20 (UTC+8)
Publisher:	SPRINGER
Abstract:	Paclitaxel has been found to exhibit cytotoxic and antitumor activity. There is little information regarding the mechanisms of apoptotic-inducing effect of pactitaxel on human osteogenic sarcoma U-2 OS cells. Several key regulatory proteins are involved in the initiation of apoptosis. Caspase-3 plays a direct role in proteolytic cleavage of cellular proteins responsible for progression to apoptosis. We examined the effect of paclitaxel on the cell cycle arrest and apoptosis in U-2 OS cells using flow cytometric analysis and Western blotting. We also measured the inhibition of paclitaxel-induced apoptosis and the caspase-3 activity by the broad-spectrum caspase inhibitor z-VAD-frnk on U-2 OS cells. The increased levels of casapse-3 were also confirmed by cDNA microarray. Our observations were: (1) paclitaxel treatment resulted in G2/M-cycle arrest in U-2 OS cells; (2) time and dose dependent apoptosis of U-2 OS cells was induced by paclitaxel; (3) in U-2 OS cells, z-VAD-tmk blocked the paclitaxel-induced apoptosis and caspase-3 activation. These results suggest that paclitaxel-induced G2/M-cycle arrest of the G2M phase and apoptosis via a caspase-3 pathway in U-2 OS cells. (c) 2005 Orthopaedic Research Society. Published by Elsevier Ltd. All rights reserved.
Relation:	RHEUMATOLOGY INTERNATIONAL 25(8):580-584
Appears in Collections:	[China Medical University Hospital] Jurnal articles

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