Investigation of the cellular mechanism of inhibition of formyl-methionyl-leucyl-phenylalanine-induced superoxide anion generation in rat neutrophils by 2-benzyloxybenzaldehyde

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題名:	Investigation of the cellular mechanism of inhibition of formyl-methionyl-leucyl-phenylalanine-induced superoxide anion generation in rat neutrophils by 2-benzyloxybenzaldehyde
作者:	Wang, JP;Chang, LC;Lin, YL;Hsu, MF;Chang, CY;Huang, LJ;Kuo, SC
貢獻者:	附設醫院基因醫學部;Taichung Vet Gen Hosp, Dept Educ & Res, Taichung 407, Taiwan;China Med Coll, Dept Biochem, Taichung 404, Taiwan;China Med Coll, Grad Inst Pharmaceut Chem, Taichung 404, Taiwan
日期:	2003
上傳時間:	2010-09-24 14:45:55 (UTC+8)
出版者:	PERGAMON-ELSEVIER SCIENCE LTD
摘要:	Renal cell injury and fixed particle formation is one of the theories of urinary stone formation. The exposure of renal epithelial cells to oxalate ions and calcium oxalate monohydrate crystals can cause free radical generation and increase lipid peroxidation. Tamm-Horsfall protein (THP) has a protective effect on the production of free radicals in vitro. We aimed to show that THP (and its deglycosylated products, D-THP) could protect culture cells from free radical injury in vivo as well as the possible mechanism by which this is done. Exposure of Madin-Darby canine kidney (MDCK) cells to Ox resulted in a significant increase in the release LDH, NBT and MDA, as well as an increase in caspase 3 activity, all of which were further elevated when COM crystals were added. With the addition of THP at 500 nM, there was a significant decrease in the release of LDH and the production of MDA and NBT. A decrease in capase 3 activity was observed when 500 nM THP was added to the culture medium that reached 32.7% and 40.4% of inhibition in CaOx + THP and CaOx + COM + THP, respectively. THP decreased the adhesion of COM crystals to the MDCK cells but lost its effect when THP was deglycosylated. The results indicate that both Ox and COM crystals cause the release of LDH, MDA, NBT and increase the activity of capase 3 in MDCK cells. As a free radical scavenger, THP reduces the amount of free radicals and provides significant protection at a critical concentration of 500 nM. The deglycosylated THP decreased the effect of the protection of the MDCK cells from oxalate-induced injury and an increase of adhesion of the COM crystals to the MDCK cells. Therefore, the effects of THP on the protection of oxalate induced radical injury may be partly due to Its intact glycosylation and its adhesion to the cell membrane.
關聯:	BIOCHEMICAL PHARMACOLOGY 65(7):1043-1051
顯示於類別:	[台中附設醫院] 期刊論文

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